Serum Apolipoprotein E Determination

Apolipoprotein E (apoE) is an important component of plasma apolipoprotein. It was first discovered in normal human very low density lipoprotein (VLDL) in 1973. It consists of a single polypeptide chain consisting of 299 amino acids. A glycoprotein with a molecular weight of 34 kD. It is of great significance for the diagnosis, prevention and treatment of hypertension, myocardial infarction, cerebral infarction and senile dementia. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) before the test, which may affect blood lipid levels. Normal value The normal reference range of 3.05-4.85 mg/dl was determined by the two-sided percentile method (95%). The male mean was 3.95±0.86 mg/dl, and the female mean was 4.00±0.91 mg/dl. There was no significant difference in gender (p>0.05). Clinical significance Abnormal results: When liver dysfunction, liver synthesis protein capacity decreased, apoE synthesis decreased, serum apoE concentration decreased. When the liver cholesterol load increases or the liver disease causes cholestatic and the plasma bile salt concentration increases, the LDL receptor is down-regulated, and the apoE-containing lipoprotein is damaged in the liver and the clearance is decreased, and the serum apoE concentration is increased. In addition, lecithin acyltransferase (LCAT) also plays an important role in lipoprotein metabolism. LCAT is synthesized by the liver, and plasma LCAT activity is decreased in liver disease, resulting in apoE-induced lipoprotein metabolism and serum apoE concentration. People who need to be examined: patients with hypertension, myocardial infarction, cerebral infarction and Alzheimer's disease. Low results may be diseases: high dementia results may be diseases: essential hypertension, hepatitis considerations Forbidden before examination: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) to affect blood lipid levels. Requirements for examination: There should be no history of acute illness, injury or surgery in the near future. Inspection process Subjects were collected blood in the fasting vein in the morning, and serum was separated in time. ApoE, apoA1, and apoB were determined by immunoturbidimetric assay; TC and TG were determined by enzymatic method; HDL-C was determined by selective inhibition method. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness.