Serum Apolipoprotein CII Determination

Apolipoprotein C (ApoCII) is one of the structural proteins of CM, VLDL and HDL, accounting for 14%, 7% to 10% and 1% to 3% of its protein components, respectively. The concentration of ApoCII in plasma is 0.03-0.05 g/L. Changes in serum Apo-CII levels may reflect lipid abnormalities earlier and more sensitively than serum cholesterol abnormalities. Active control of lipid metabolism disorders plays an important role in the prevention and treatment of cerebral infarction. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) to affect blood lipid levels. Normal value The minimum detection amount is 25 ng, the standard curve working range is 1.5 to 30.0 mg, the intraplate and interplate variation coefficients are 6.5 to 7.8% and 6.6 to 11.0%, respectively, and the recovery rate is 107.5%; 185 normal human serum apoCII content, male 5.1 ± 1.9 mg% (n = 95), female 4.8 ± 1.7 mg% (n = 90). Clinical significance Abnormal results: ApoCII deficiency is an autosomal recessive disorder. Plasma ApoCII levels in heterozygotes are only half of normal, plasma triglyceride concentration can still maintain normal, homozygous plasma ApoCII is completely lacking, although LPL structure is normal, plasma ApoCII is still up to 10g / L, showing type I high lipoprotein Hypertension can cause hepatosplenomegaly in severe cases and induce acute pancreatitis. People who need to be examined: patients with hyperlipoproteinemia. High results may be diseases: high blood lipids, hyperlipoproteinemia type II precautions Forbidden before examination: It is forbidden to take certain drugs (such as birth control pills, thyroid hormones, steroid hormones, etc.) to affect blood lipid levels. Requirements for examination: There should be no history of acute illness, injury or surgery in the near future. Inspection process Analysis of the terminal amino group is one of the methods for identifying the purity of the protein. A protein of a peptide chain, through the quantitative analysis of the N-terminus, should have an integer number of N-terminal amino acids per gram of protein, and the presence of a small amount of other terminal amino acids often indicates the presence of impurities. Analysis of the protein's various amino acid percentages can also be used as a reference for the identification of various proteins. The final purity standard should be the determination of the amino acid sequence. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness.