serum free fatty acids

Free fatty acids, also known as nonestesterified fatty acids (NEFA), are rarely present in serum. For example, sensitive methods must be used for the determination of small amounts of serum samples, and the interference of fatty acids produced by fat hydrolysis should be avoided. Reduce thyroid dysfunction, Addison's disease, islet cell tumor, pituitary hypofunction, hypoglycemic agents or excessive use of insulin. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: After 8 pm on the day before the medical examination, you should fast, so as not to affect the detection of indicators such as blood glucose in the second sky. Normal value Enzymatic method (37 ° C) 400 ~ 900 μmol / L. Clinical significance (1) High serum free fatty acids: diabetes, glycogen accumulation disease, hyperthyroidism, brown cell tumor, acromegaly, giant disease, Cushing's syndrome, severe liver damage, myocardial infarction, late pregnancy, obstructive jaundice , hepatitis, cirrhosis, hemochromatosis, etc. (2) low serum free fatty acids: hypothyroidism, Addison's disease, islet cell tumor, pituitary hypofunction, hypoglycemic agents or excessive use of insulin. Low results may be diseases: hereditary dysmotility, polyneuritis, high results, possible diseases: myocardial infarction If the serum free fatty acid concentration is greater than 2mmol / L, it can be diluted after appropriate dilution. Inspection process (1) Mixing reagents: 1 Reagent I: buffer solution I4.0ml, ATP2.0ml, MgCl2.0ml, coenzyme A2.0ml, TritonX-100-4.0ml, acyl-CoA synthetase 2.0ml, plus double distilled water 4.5ml (total 20.5ml) . 2 Reagent II: containing MES buffer 10.0ml, 4-aminoantipyrine 6.0ml, TBHB8.0ml, NaN3 solution 2.0ml, TritonX-100-4.0ml, peroxidase solution 0.4ml, double distilled water 10.0ml (Total 40.4ml). 3 Reagent III: 2.1 ml of NEM buffer, 2.0 ml of acyl-CoA oxidase (4.1 ml in total). The above three mixed reagents can be stable for 3 days at 4 ° C in the dark. (2) Add 1 ml of reagent to 2 ml of reagent II in each tube and mix at 37 ° C for 10 min. (3) Add 50 μl of blank and serum samples, mix at 37 ° C for 5 min, and measure the absorbance at 546 nm to A1. (4) Add 0.20 ml of the reagent III, mix, and wait for 20 to 25 minutes to stop the reaction and measure the absorbance at 546 nm to A2. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness.