Serum Intrinsic Factor Blocking Antibody Test

The serum factor-blocking antibody test uses the RIA method to detect whether there is a serum factor blocking antibody in the blood sample. The inner factor blocking antibody can block the binding of the inner factor to the vitamin B12 and affect the absorption of vitamin B12. The detection rate of blocking antibody (IFBA) in the serum of patients with pernicious anemia is more than 50%, which can be used as one of the screening methods for pernicious anemia. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: After 8 pm on the day before the medical examination, you should fast, so as not to affect the detection of indicators such as blood glucose in the second sky. Normal value The RIA method is negative. Clinical significance Abnormal results: Positive: IFBA is mainly seen in patients with pernicious anemia. The detection rate of type I was 32.8% to 70.1% positive; type II was 11.5% to 42.9% positive; the simultaneous positive rate of both types was 24.0% to 34.0%. In patients with pernicious anemia, the I type IFA titer is about 221 units, and the type II is about 53 units. IFA titers are usually not associated with disease severity or length of disease. The detection rate of other diseases IFBA is 1% to 3% for diabetes, 0.7% to 4.7% for hyperthyroidism, 3% for chronic thyroiditis, and 2% for iron deficiency anemia. The positive rate of normal people is <1%. IFBA can cause gastric mucosal atrophy, no gastric acidity, inhibition of internal factor activity and decreased blood vitamin B12 concentration, leading to pernicious anemia and neurological symptoms. The internal factor antibody belongs to IgG and can be transmitted to the newborn through the placenta. IFBA can be measured in the blood and gastric juice of the baby 1 to 3 months after birth. People who need to be examined: people with anemia. Positive results may be diseases: iron deficiency anemia, anemia considerations Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Blood samples were taken for RIA examination. Not suitable for the crowd A patient with hemophilia and severe clotting factor deficiency. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.