serum unsaturated iron binding capacity

The content of high iron is measured by the method of measuring serum iron. This amount is called total iron binding force (TIBC), and the serum iron value is subtracted from TIBC, which is called unsaturated binding force. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Before using blood, carefully treat with iron or fast foods with high iron content, such as animal liver. Normal value 25.06 to 51.91 μmol/L (140 to 290 μg/dl). Clinical significance See Table 2. Precautions (1) Serum iron was highest in the morning of 1 day and gradually decreased in the afternoon. Specimens should be taken on an empty stomach in the morning. (2) Before the blood test, use iron to treat or fast foods with high iron content, such as animal liver; fasting energy and iron complex substances, such as tea. (3) When women in the menstrual period, pregnancy and infant growth period, due to increased iron requirements in the body, serum iron can be reduced, which is a physiological phenomenon. Inspection process Take 1 ml of serum (pulp), add 1 ml of iron standard application solution, mix, place for 5 min, add 200 mg of magnesium carbonate powder, mix, set at room temperature for 30 min, and mix once every 10 minutes. Centrifuge at 2500 rpm for 10 min and aspirate the supernatant for operation. Mix, boil in a boiling water bath for 5 min, cool to room temperature, centrifuge to precipitate, take the supernatant (if not clear chloroform 1ml to remove fat), use 530nm or green filter colorimetric, adjust the absorbance to 0 point with a blank tube, read Take the absorbance of each tube. Not suitable for the crowd There are no special taboos. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.