Plasma Endothelin Determination

The plasma endothelin assay measures endothelin in plasma and has an important diagnostic role in the pathogenesis of hypertension and cerebrovascular accident patients. The test results were positive, that is, plasma endothelin was greater than 1.0pg/ml, suggesting that there may be diseases such as cardiovascular, respiratory, digestive, and nervous systems. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Check the use of venous blood, relax the mood when taking blood, avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Normal value Healthy human plasma endothelin ET was 1.0 pg/ml. Clinical significance Abnormal result The test results were positive, that is, plasma endothelin was greater than 1.0pg/ml, suggesting that there may be diseases such as cardiovascular, respiratory, digestive, and nervous systems. People who need to be examined are suspected of having heart and blood, breathing, digestive, and neurological diseases. Positive results may be diseases: cardiovascular disease precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: Check the use of venous blood, relax the mood when drawing blood, avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Inject 30 μl of 10% EDTA disodium and 40 μl of aprotinin into a test tube, centrifuge at 10 ° C for 10 min, and rotate at 1500 r/min. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.