hemoglobin resistance test

The ability of HbF to resist alkali denaturation is stronger than that of HbA2, and in an alkaline solution, other Hb is variably precipitated in an alkaline solution and precipitated by a precipitant. HbF is not easily denatured and precipitated, and the Hb content in the filtrate is determined as the content of HbF. The content can be determined by colorimetry. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: The diet is light before the test. Check for an empty stomach in the morning. Normal value See Table 1. Clinical significance (1) Increased α, β, A2F, γ-thalassemia (δ-thalassemia does not increase). (2) mildly increased acute leukemia, aplastic anemia, erythroleukemia, malignant tumors. Precautions (1) If the filtrate is light yellow or reddish, it may be high in hemoglobin content, but first check the quality of the lye and acidic semi-saturated ammonium sulfate used, due to insufficient alkalinity of the lye or the concentration of acidic semi-saturated ammonium sulfate. Insufficient acidity can result in the filtrate not being transparent to water and being yellowish, reddish, etc. The result is erroneously increased. (2) The concentration of lye must be accurate, and its pH must be greater than 12. After calibration, it is best to keep the small part sealed and stored, the amount of use and the action time must be very accurate. (3) Acidic semi-saturated ammonium sulfate must be accurately prepared, and its pH should be 3.0, preferably in small batches. (4) The filter paper should be a chemical test article. The model must be stable and the filtrate must be clear and transparent, otherwise it should be re-filtered once or centrifuged. (5) Test tubes, pipettes and other instruments used in the test should not contaminate acid and alkali. (6) It is best to repeat two copies for each test. Inspection process (1) 1.6 ml of 0.083 mol/L KOH was added to the test tube, and a 25 ° C water bath was placed. (2) After 5 to 10 minutes, add 0.1 ml of hemoglobin solution, immediately start the stopwatch, wash 6 times with a straw, and gently shake the tube for 10 s. This step needs to be completed within 20s. (3) After accurate timekeeping for 60 s, add 3 ml of acidic ammonium sulfate solution, and immediately mix and invert 6 times. (4) Filtered with filter paper, the filtrate was subjected to colorimetry at a wavelength of 540 nm, and distilled water was used as a blank to read the absorbance of the anti-alkaline hemoglobin. (5) Another 0.02 ml of hemoglobin solution was taken, added to 5 ml of water, mixed, and colorimetrically read according to the above method to read the absorbance of total Hb. (6) Calculation of results: The dilution of the alkali-resistant Hb tube was 1:51, and the dilution of the total Hb tube was 1:251, and the ratio was 51:251=0.203. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Infection: Do not touch the dirt after blood collection. Do not wash your hands immediately to avoid infection.