fibrinogen

Plasma fibrinogen is a macromolecular globulin synthesized in the liver with a molecular weight of 340,000, which is associated with plasma coagulation. Fibrinogen produces fibrin monomer by thrombin during coagulation. The latter is polymerized and converted to fibrin filaments in the presence of XII and calcium ions to form a network of intertwined fibrin, which surrounds red blood cells, white blood cells and platelets to form a blood clot. Commonly used measurement methods include fibrin colorimetry, thrombin coagulation time method, salting out separation colorimetry, salting out separation turbidimetry and the like. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Take blood for 12 hours and take fresh blood for inspection. Normal value The biuret colorimetric method is 2 to 4 g/L (200 to 400 mg/dl). Clinical significance (1) increase 1 Physiological elderly, late pregnancy, oral administration of estrogen preparations, exercise, etc. 2 acquired gestational toxicosis, infectious diseases, malignant tumors, cerebral infarction, myocardial infarction, surgery, collagen disease, diabetes, nephrotic syndrome, blood preparation containing fibrinogen, long-term or large-scale input (AHG anti-human globulin preparation, Fresh frozen plasma) and so on. (2) reduction 1 physiological newborn. 2 congenital A. no fibrinogenemia. B. Low fibrinogenemia. C. Part of abnormal fibrinogenemia. 3 acquired nature A. The formation of disorders chronic hepatitis, cirrhosis. B. Consumption increases diffuse intravascular coagulation (DIC), thrombosis, major bleeding, use of snake venom preparations, and the like. C. Fibrinolysis, shock (shock), surgery (primary fibrinolysis), DIC (secondary fibrinolysis), etc. Low results may be diseases: amniotic fluid embolism, disseminated intravascular coagulation, high myocardial infarction may be disease: cerebral infarction, cirrhosis, chronic hepatitis precautions 1. Take blood for 12 hours and take fresh blood for examination. 2, oral contraceptives can increase fibrinogen. 3, women's menstrual period, pregnancy, fibrinogen can also rise. 4, fibrinogen suddenly increased, often indicating the possibility of tumor metastasis. Inspection process (1) Take a blood sample tube with a 2ml scale, add 109mmol/L sodium citrate 0.2ml, then add about 2ml of venous blood to the mark and shake. The plasma was separated by centrifugation for 10 min. (2) Take 1 tube of 10×100mm small tube and add 0.2ml of plasma, then add 0.2ml of 36mmol/L calcium chloride solution or 0.2ml of thrombin, mix and set in a water bath at 37°C for 1h. (3) Lightly shake the small test tube to loosen the fibrin clot. Insert a small glass rod along the wall of the tube to gently press the fibrin clot, slowly release the fibrin, and finally roll the fibrin onto the small glass rod. (4) Wash the fibrin piece 4 times with water. (5) Take 2 tubes of 15×150mm and write the measuring tube (U) and the blank tube (B). (6) Put the fibrin into the U tube, add 0.2 ml of 2.5 mol/L sodium hydroxide to each of the U and B tubes, and then heat the U tube in a boiling water bath at 100 ° C for 5 min until the fibrin is completely dissolved. (7) Add about 5 ml of water and 1.0 ml of phenol reagent to each of the 2 tubes, add water to 10 ml each, and finally add 3 ml of each of 1.9 mol/L sodium carbonate solution. After standing at room temperature for 30 min, the B tube was used as a blank tube, and the absorbance of the U tube was read at a wavelength of 580 to 640 nm, and the standard curve of the pre-painted standard was used to obtain the plasma fibrinogen concentration result. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.