Reticulocyte count and reticulocyte percentage

Reticulocytes are transitional cells between young red blood cells and fully mature red blood cells, slightly larger than mature red blood cells, and have a diameter of about 8 to 9.5 μm. Due to the presence of basophilic RNA in the plasma, after in vivo staining with Huang tar blue, new methylene blue, and sodium sulphate dye solution, a blue-green network structure can be seen in the cytoplasm. Red blood cells. After 24 to 48 hours in the blood circulation, the remnant basophils are gradually eliminated and become mature red blood cells. Heilmyer divides reticulocytes into 5 types according to their different developmental stages. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Keep a normal mindset. Normal value Microscopic counting Ret (24 to 84) × 109 / L (24000 to 84000 / mm3). The percentage of newborns is 0.03 to 0.06 (3% to 6%). Children and adults 0.005 to 0.015 (0.5% to 1.5%). Clinical significance (1) Increased hemolytic anemia, acute massive hemorrhage, iron deficiency anemia and megaloblastic anemia have significant therapeutic effects. (2) reduce aplastic anemia (slightly increased in chronic), some hemolytic anemia in the case of aplastic crisis. Precautions (1) In recent years, ICSH and the National Committee for Clinical Laboratory Standards (NCCLS) define reticulocytes: reticulocytes do not contain nucleus, but with new methylene blue staining, it can still be found to contain ribonucleic acid. At least two or more red blood cells of the particles. (2) One method of counterstaining has a tendency to decrease, and the second method of counterstaining makes the particles difficult to distinguish, so the reticulocyte count is not counterstained. Inspection process (1) The hematocrit is measured while measuring the number of reticulocytes in the patient. (2) Determination of the ripening time of reticulocytes in the blood. (3) The reticulocyte production index was determined according to the following formula. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.