acid hemolysis test

Acid hemolysis test, also known as Ham test. It means that the patient's red blood cells act in the environment of 37 ° C together with the acidified serum, and the red blood cells are destroyed, which is positive. Positive in paroxysmal nocturnal hemoglobinuria, hereditary spherocytosis, autoimmune hemolytic anemia. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Found in paroxysmal nocturnal hemoglobinuria. Tips: Before taking blood, please explain whether you are taking oxygen. Normal value negative. Clinical significance The acid hemolysis test is the main diagnostic test for the diagnosis of paroxysmal nocturnal hemoglobinuria. Positive in paroxysmal nocturnal hemoglobinuria, hereditary spherocytosis, autoimmune hemolytic anemia. Positive results may be diseases: pediatric paroxysmal nocturnal hemoglobinuria precautions (1) Notes on blood draw: such as fasting. (2) Negative results can not rule out paroxysmal nocturnal hemoglobinuria, because about 25% of patients with this disease have poor bone marrow hyperplasia, lack of complement-sensitive red blood cell production, patients can have no hemoglobinuria for a period of time, so acid hemolysis The test is negative and should be reviewed or supplemented several times. (3) Hemolysis should be absolutely avoided when collecting blood. (4) PNH patients who received a large number of blood transfusions before the test were normal because the imported red blood cells were normal, so the results were negative. Inspection process (1) 10 ml of blood of a healthy person having the same blood type as the subject was collected intravenously. Approximately 8 ml was placed in a common test tube for separation of serum. 2 ml was anticoagulated with heparin for separation of red blood cells. The red blood cells were washed twice with physiological saline at the time of separation to prepare a 50% red blood cell suspension. (2) 2 ml of blood was collected from the subject, and heparin was anticoagulated. The red blood cells were washed twice with physiological saline to prepare a 50% red blood cell suspension. (3) Place the healthy human serum in 6 small tubes, 0.5 ml per tube. Two of them were placed in a 56 ° C water bath for 1 h, which was inactivated serum. The remaining 4 were placed at room temperature. (4) According to the procedure, 0.2 mol/L hydrochloric acid, a patient or a healthy human 50% red blood cell suspension was added. (5) All tubes were incubated at 37 ° C for 1 h. The precipitate was centrifuged, and the degree of hemolysis of the supernatant was observed. Perform colorimetry when necessary and calculate the hemolysis rate (%). Not suitable for the crowd A patient with hemophilia and severe clotting factor deficiency. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.