Sodium dodecyl sulfate Hb determination method

The Hb measurement is roughly divided into 1 according to the Hb molecular composition, and the total Hb method (whole blood iron method) is measured. 2 According to the physical characteristics of blood, Hb (specific gravity method, refractometer method). 3 According to the characteristics of the reversible binding of Hb and O2, Hb (blood gas fractionation method) was measured. 4 According to the spectral characteristics of Hb derivatives, four kinds of quantitative determination, some of which are simple and easy to use, and have been widely used for a long time (such as Shali method), but with the advancement of technology and research, the shortcomings become more and more obvious. Gradually eliminated. In order to unify the Hb measurement method, the International Hematology Standardization Committee recommended the cyanide high-iron Hb measurement method as the Hb measurement standard method in 1966. The HiCN method was reiterated in an international document published jointly by the International Federation of Clinical Chemistry and the World Pathology Society in 1978. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Please relax when checking. Normal value Adult males (120-170) g/L, adult females (110-150) g/L, neonates (170-220) g/L. Clinical significance (1) increase 1 physiological changes in neonates, residents in high mountains, strenuous physical labor or sports, super-powered people. 2 pathological changes A. Polycythemia (secondary or relative) a large number of dehydration caused by blood concentration (severe vomiting, frequent diarrhea, excessive sweating, polyuria, severe burns, long-term fasting), long-term hypoxia (purpuric congenital Heart disease, pulmonary heart disease, chronic mountain disease, severe emphysema, chronic carbon monoxide poisoning. B. Polycythemia vera. (2) reduction 1 physiological changes in the middle and late stages of pregnancy. 2 pathological changes A. Hemorrhagic anemia visceral hemorrhage, hemophilia, thrombocytopenic purpura and the like. B. Hemolytic anemia congenital hemolytic anemia (thalassemia, faba bean disease, congenital non-spherical red blood cell anemia, congenital hemolytic jaundice, etc.), acquired anemia (autoimmune hemolytic anemia, neonatal hemolytic disease, array Primary sleepy hemoglobinuria, red blood cell debris syndrome, drug-induced hemolytic anemia quinine, quinidine, phenacetin, etc., toxic hemolytic anemia benzene, lead, arsenic, etc., infectious hemolytic anemia). C. Hematopoietic anemia, iron deficiency anemia, pernicious anemia, malnutrition anemia, aplastic anemia, etc. Precautions The advantages of this method are simple operation, stable color, accuracy and accuracy in line with requirements, and no pollution. It is recommended as a secondary method in the National Conference on Clinical Laboratory Methods. However, the quality of SDS itself is quite different and SDS destroys white blood cells and is not suitable for use in blood cell analyzers. Inspection process Same spectrophotometer method. Not suitable for the crowd no. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.