Ristomycin-induced platelet aggregation assay

The ristocetin-induced platelet aggregation assay is to add ristocetin to platelet-rich plasma (PRP), platelet aggregation, plasma turbidity change, and increased transmittance. According to the principle of electrical impedance, by immersing and recording A method of determining platelet aggregation in a whole blood sample by measuring a change in minute current or impedance between electrode probes in a whole blood sample. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The normal value is 20-40. Clinical significance Abnormal result 1. Increased platelet aggregation reflects enhanced platelet aggregation. Found in hypercoagulable state and/or prethrombotic state and thrombotic diseases such as myocardial infarction, angina pectoris, diabetes, cerebrovascular disease, pregnancy induced hypertension syndrome, venous thrombosis, pulmonary infarction, oral contraceptives, late pregnancy, high fat Blood, antigen-antibody complex reaction, artificial heart and valve transplantation. 2. Decreased platelet aggregation reflects a decrease in platelet aggregation. Seen in the acquisition of reduced platelet function such as uremia, cirrhosis, MDS, primary thrombocytopenic purpura, acute leukemia, taking antiplatelet drugs, low (no) fibrinogenemia. Also seen in hereditary platelet function defects. People who need to be examined have a heart disease, a blood disease. Low results may be diseases: uremia, liver cirrhosis results may be high disease: myocardial infarction, diabetes precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. The measurement should be completed within 3 hours after the blood collection. If the time is too long, the aggregation intensity and speed will decrease. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Dizziness or fainting: When blood is drawn, due to emotional overstress, fear, reflex caused by vagus nerve excitability, blood pressure drop, etc., the blood supply to the brain is insufficient to cause fainting or dizziness.