spherocytes

Spherocytes are common in hereditary spherocytosis and other hemolytic anemias with spherocytosis, such as autoimmune hemolytic anemia, neonatal hemolytic disease, and hemolytic anemia caused by erythrocyte enzyme defects. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: venous blood collection, long-term ligation of the tourniquet can cause false increase, the straw is unclean, not accurate, will count or increase or decrease, absorb the dilution, now use a manual dosing device, should calibrate the capacity before use . Normal value 2.5% to 3.0%. Clinical significance Increased in hereditary spherocytosis (>30%, heavy 100%), autoimmune hemolytic anemia, abnormal hemoglobin disease, cirrhosis and hypersplenism, chronic infectious diseases, heminoid hemolytic. High results may be diseases: congenital erythrocyte disease, oral polycythemia considerations An increase in the count amount can relatively reduce the error. The red blood cell count decreased from 5 pm to 7 am and after meals, up to 10%. Bed rest can be reduced by 5.7% compared with normal activities. Therefore, time, diet and time should be properly considered when evaluating two blood test results. Bed rest and other factors. Red blood cell counts are highly prone to errors. Venous blood collection, long-term ligation of the tourniquet can cause false increase, the pipette is unclean, not accurate, will count or increase or decrease; draw the diluent, now use a manual dosing device, the capacity should be calibrated before use; Try to buy the exact one; count enough when counting. If you pay attention to it, the results obtained by the manual method still have good accuracy and can be used to correct the automatic counter. Inspection process (1) Take one tube and add 4 ml of the diluent. (2) Pipette 20 μl of peripheral blood with a calibrated micropipette. (3) Wipe off the remaining blood outside the pipette, blow 20μl of whole blood into the diluent, and wash the remaining blood in the pipette with the diluent, and mix immediately. (4) Wipe the counting plate and the cover glass and cover the cover glass on the counting plate. (5) Drain the mixed red blood cell suspension into the counting chamber with a pipette. (6) After standing for 2 to 3 minutes, use the high power microscope to count the cells on the red blood cell line on the four squares in the central square and the five squares in the middle, according to the principle of “no number, no number, no right”. count. The count error of each square in normal blood is not more than 10. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.