Ferric cyanide Hb determination method

The Hb measurement is roughly divided into: 1 The total Hb method (whole blood iron method) is determined based on the molecular composition of Hb. 2 According to the physical characteristics of blood, Hb (specific gravity method, refractometer method). 3 According to the characteristics of the reversible binding of Hb and O2, Hb (blood gas fractionation method) was measured. 4 According to the spectral characteristics of Hb derivatives, four kinds of quantitative determination, some of which are simple and easy to use, and have been widely used for a long time (such as Shali method), but with the advancement of technology and research, the shortcomings become more and more obvious. Gradually eliminated. In order to unify the Hb measurement method, the International Hematology Standardization Committee recommended the cyanide high-iron Hb measurement method as the Hb measurement standard method in 1966. The HiCN method was reiterated in an international document published jointly by the International Federation of Clinical Chemistry and the World Pathology Society in 1978. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Can be seen in hemorrhagic anemia visceral bleeding, hemophilia, thrombocytopenic purpura and so on. Normal value: Adult male: 120-170 Adult female: 110-150 Newborn: 170-220 Above normal: Increased in polycythemia. negative: Positive: Tips: The HiCN method is listed as an international reference method for Hb determination. However, there are still some problems in practical applications. The fatal point is that KCN is highly toxic, and improper management can cause public hazard, so be sure to pay attention when using it. Normal value Adult males (120-170) g/L, adult females (110-150) g/L, neonates (170-220) g/L. Clinical significance (1) increase 1 physiological changes in neonates, residents in high mountains, strenuous physical labor or sports, super-powered people. 2 pathological changes A. Polycythemia (secondary or relative) a large number of dehydration caused by blood concentration (severe vomiting, frequent diarrhea, excessive sweating, polyuria, severe burns, long-term fasting), long-term hypoxia (purpuric congenital Heart disease, pulmonary heart disease, chronic mountain disease, severe emphysema, chronic carbon monoxide poisoning. B. Polycythemia vera. (2) reduction 1 physiological changes in the middle and late stages of pregnancy. 2 pathological changes A. Hemorrhagic anemia visceral hemorrhage, hemophilia, thrombocytopenic purpura and the like. B. Hemolytic anemia congenital hemolytic anemia (thalassemia, faba bean disease, congenital non-spherical red blood cell anemia, congenital hemolytic jaundice, etc.), acquired anemia (autoimmune hemolytic anemia, neonatal hemolytic disease, array Primary sleepy hemoglobinuria, red blood cell debris syndrome, drug-induced hemolytic anemia quinine, quinidine, phenacetin, etc., toxic hemolytic anemia benzene, lead, arsenic, etc., infectious hemolytic anemia). C. Hematopoietic anemia, iron deficiency anemia, pernicious anemia, malnutrition anemia, aplastic anemia, etc. Low results may be diseases: high hemophilia results may be diseases: secondary erythrocytosis considerations The HiCN method has the advantages of simple operation, fast color development and stability (how to save the color after 6 years of non-fading), and all kinds of Hb except SHb can detect and read the absorbance and directly set the value. The key to determining the accuracy of the measurement is the calibration of the instrument. In actual work, the spectrophotometer used is difficult to meet the standard requirements, and the K value is often used to correct the result. It is important to check the K value periodically. The HiCN method is listed as an international reference method for Hb determination. However, there are still some problems in practical application. The fatal point is that KCN is highly toxic. Poor management can cause public hazards. In addition, high white blood cells and hyperglobulinemia can cause turbidity of reagents, and the problem of slower conversion of HbCO is not complete. solve. Inspection process HiCN method. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.