immunosuppressive acidic protein

Immunosuppressive acidic protein (IAP) is a subclass of α1-acid glycoprotein sugar chain structure. Mainly produced in hepatocytes, macrophages and granulocytes, its relative molecular mass is 50kD, isoelectric point is 3.0, containing 31.5% of carbohydrates; in vivo it can inhibit the humoral and cellular immune functions of tumor-bearing hosts. Basic Information Specialist classification: Oncology examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value Serum 237 ± 106 mg / L. Clinical significance Abnormal result (1) The serum IAP of lung cancer patients is significantly higher than that of healthy people and benign lung diseases, indicating that serum IAP level can be used as a good indicator to identify benign and malignant diseases of the lung. The detection of serum IAP level has certain clinical significance for the estimation of preoperative cellular immune function and postoperative treatment of lung cancer patients. (2) Serum IAP levels in patients with ovarian cancer were significantly higher than those in healthy women and benign ovarian tumors. The IAP value increases with increasing ovarian cancer stage. Serum IAP levels were significantly decreased after chemotherapy; serum IAP levels were significantly increased after recurrence. It indicates that serum IAP level can be used as a useful indicator for disease monitoring, treatment response and prognosis of ovarian cancer patients. Serum lAP values ​​in patients with cervical cancer and endometrial cancer can also be significantly increased. (3) When acute pancreatitis, acute thyroiditis and other diseases, the IAP value will also increase, but most of them are transient increases. High results may be diseases: precautions for metastatic pleural tumors Requirements for inspection: (1) The incubation temperature and time must be strictly controlled during the test in order to reach the end of diffusion. In the test diffusion process, it is especially necessary to prevent sudden changes in temperature to avoid double circles. (2) After the sample is placed in the wet box, the agar plate must be kept in a horizontal position to prevent the eccentricity of the sediment ring. Preparation before inspection: (1) It must be fasted within 8 hours before blood draw. (2) Do not eat too greasy, high-protein foods the day before the inspection to avoid heavy drinking. Not suitable for the crowd: there is no inappropriate crowd. Inspection process The IAP kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards of known IAP concentrations and samples of unknown concentration are added to the microplates for detection. IAP and biotinylated antibodies were first incubated simultaneously. After washing, avidin-labeled HRP was added. After incubation and washing, the unbound enzyme conjugate is removed, then the substrates A, B, and the enzyme conjugate are added simultaneously. Produce color. The depth of the color is proportional to the concentration of IAP in the sample. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.