Treponema Activity Test

Treponemapallidum (TP) is a genus of Treponema genus, which is the pathogen of human syphilis. Syphilis is one of the main sexual diseases of human beings. There are two types of antibodies in the serum of syphilis patients: one is a reactive enzyme, which is a mixed antibody of IgA and IgM type, which can react non-specifically with cardiac lipid antigen extracted from mammalian myocardium, and has no protective effect on the body, but is available for serology. For diagnostic screening tests. The other type is a Treponema pallidum-specific antibody that inhibits the movement of live Treponema pallidum under anaerobic conditions and in the presence of complement, and can kill or dissolve it, which can be used to confirm the syphilis. The cultivation of Treponema pallidum is very difficult, so it is mainly diagnosed by serological diagnosis. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive Treponema infection. Reminder: After 8:00 pm on the previous day, you should fast, so as not to affect the next day's test. Normal value negative. Clinical significance Abnormal results: The sensitivity and specificity of this test are high, but the operation is complicated, and it is rarely used clinically. It is only used for the diagnosis and research work of difficult patients. Positive Treponema infection. Need to check the crowd: A group of people who have experienced unclean sex life. Positive result may be disease: syphilis precautions Requirements for inspection: 1, when the blood test, there are symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of sugar water, until the symptoms are relieved. 2, early syphilis first appeared positive, specificity of 94% to 100%. Preparation before inspection: 1. After 8:00 pm on the day before the inspection, fasting should be done to avoid affecting the test of the next day. Not suitable for people: 1. There are no unsuitable people. Inspection process The collection of pathogen detection specimens takes blood within one week of onset, urine is taken in the second week, and cerebrospinal fluid is taken from meningeal irritation, and the detection rate is higher. 1. Direct microscopy The specimens are subjected to dark-field microscopy after differential centrifugation or by Fontana silver-plated staining microscopy. They can also be examined by direct immunofluorescence or immunoenzymatic staining. 2. Isolation culture and identification The specimens were inoculated in Korthof medium and cultured at 28 °C for 2 to 4 weeks. If there was growth, the medium became cloudy, and then the dark field microscopy was used to check the presence of leptospira. Learn how to identify its group. 3. Animal inoculation is a sensitive method for separating leptospira, especially for specimens contaminated with bacteria. The method is to inoculate the specimen into the abdominal cavity of young and hamsters. After 3 to 5 days of inoculation, the peritoneal cavity fluid was examined with a dark field; the heart blood was examined and isolated and cultured. After the animal is dissected after death, there are a large number of leptospirosis in the lungs and subcutaneous hemorrhage spots, liver and spleen. 4. Detection of nucleic acid method Isotopic or biotin-labeled DNA probes for nucleic acid hybridization and PCR methods can be used to detect the nucleic acid of Leptospira in the specimen for diagnosis. The specificity and sensitivity are superior to the culture method, and the results are obtained. fast. In addition, restriction endonuclease fingerprints can also be used for strain identification and typing of Leptospira. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.