basic embryonic protein

Basicfetoprotein (BFP) is a fetal protein found in fetal serum and intestinal tissues. There are molecular weights of 73 kD in various cancer tissues. Hepatic dysfunction, stomach pain, and abnormal organ pain in the population to be tested. Basic Information Specialist classification: Digestive examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Fasting for 12 hours before taking blood, taking fresh blood for inspection. Normal value Serum <80 μg / L. Clinical significance Abnormal result The increase of alkaline fetal protein is seen in primary liver cancer, cholangiocarcinoma, gallbladder cancer, pancreatic cancer, and secondly, it has certain significance for the judgment of clinical anticancer effect of gastric cancer and liver cancer and the monitoring of postoperative recurrence. Benign diseases also have a certain increase, and the positive results should be combined with clinical analysis. People who need to be tested Abnormal liver function, stomach pain, abnormal digestion, organ pain. Precautions Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. Fasting for 12 hours before taking blood, taking fresh blood for inspection. When checking: When you draw blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Not suitable for the crowd: no. Inspection process (1) The one-step enzyme labeling method is used to detect the supply of the alpha-fetoprotein kit. The method adds the sample and the enzyme label together and incubates for 1 hour, and the operation is simpler and faster. (2) Alpha-fetoprotein dot enzyme immunoassay (DEIA) detection, the operation point is to add 2μl of anti-alpha-fetoprotein antibody to the nitrocellulose membrane, dry at room temperature for 15min, and blocked with 10g/L bovine serum albumin for 1h, using filter paper After blotting, add 2 μl of serum to be tested, set at room temperature for 30 min, wash the membrane 3 times with a washing solution containing 1% Tween-20, blot dry, add anti-AFP antibody-enzyme label, and incubate at 37 ° C for 1.5 h. The membrane was washed 4 times, and the substrate was added for 5 to 10 minutes. Then, the membrane was immersed in water to terminate the reaction, and the coloring spots appeared to be positive at the loading site. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Infection: Do not touch the dirt after blood collection. Do not wash your hands immediately to avoid infection.