Toluidine blue correction test

The toluidine blue corrective test is a test that uses toluidine blue to correct the prolonged thrombin time to detect whether heparin and heparin-like substances are present in the blood. People who need to be examined often have nausea, vomiting, loss of appetite, fatigue, and sometimes pain in the liver area (upper right abdomen). Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Found in the use of heparin, nitrogen mustard and anaphylactic shock, severe liver disease, disseminated intravascular coagulation, hepatectomy, liver transplantation and so on. Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The result of the test was negative. Clinical significance Abnormal result The test result was positive. The clotting time was shortened after adding toluidine blue, and the free heparin time was negative (prolonged thrombin time, and the thrombin time was shortened by more than 5 s after adding toluidine blue). It was found in heparin, nitrogen mustard and anaphylactic shock. Severe liver disease, disseminated intravascular coagulation, hepatectomy, liver transplantation, etc. People who need to be examined often have nausea, vomiting, loss of appetite, fatigue, and sometimes pain in the liver area (upper right abdomen). Positive results may be diseases: mucopolysaccharidosis, acquired circulatory anticoagulant compound syndrome Inappropriate crowd: None. Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. After taking a blood sample, it was allowed to stand, and then toluidine blue was added to observe the clotting time. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.