Red blood cell mechanical fragility test

Normal human blood only needs sterile blood collection to make defibrinated blood. After incubation at 37 ° C for 24 to 48 hours, slight hemolysis will gradually occur. After taking out, the colorimetric rate is calculated as in the quantitative method, and the hemolysis rate (percentage) is calculated. If the person in need of examination has chronic progressive anemia, pale, hepatosplenomegaly, dysplasia, often mild jaundice and other symptoms, or family members with thalassemia. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value 0.075 to 0.24 (7.5% to 24%). Clinical significance Abnormal result (1) Hereditary spherocytosis, mechanical fragility test The degree of hemolysis is higher than the osmotic fragility test. (2) Thalassemia increased resistance to permeability and weakened to mechanical brittleness test. If the person in need of examination has chronic progressive anemia, pale, hepatosplenomegaly, dysplasia, often mild jaundice and other symptoms, or family members with thalassemia. Low results may be diseases: hereditary spherocytosis results are high. Possible diseases: thalassemia considerations Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The examination process: blood is drawn, blood is taken for examination, and venous blood is generally taken, and blood is drawn by a doctor or a nurse. The amount of blood drawn is determined according to the difference of the test content and the number of items. The blood volume is usually 2-20 ml, and the maximum is not more than 50 ml. Then the doctor takes the quantitative color for colorimetry and calculates the hemolysis rate. . Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.