red blood cell deformability

Red blood cell deformability is one of the important factors affecting the apparent viscosity of blood and the effective perfusion of microcirculation in vivo. At the same time, it is an important determinant of red blood cell life. Red blood cell deformability is determined by cell internal factors such as viscoelasticity of the cell membrane, viscosity (internal viscosity) of the cytoplasm, and cell geometry. In addition, external factors such as flow field shear stress, pH, osmotic pressure, temperature also have an effect on red blood cell deformation, so these external factors should be controlled when measuring red blood cell deformation. At present, there are many methods for measuring the deformability of red blood cells, which can be basically divided into two categories, the first type using red blood cell suspension, indirectly estimating the average deformability of the red blood cell population. Such as viscosity measurement, reverse rotation rheometer measurement, microfiltration, laser diffraction, etc.; the second is the use of a single red blood cell to determine its deformability and mechanical properties of the cell membrane, such as the bottom surface difference method, micropipette Method, electron spin resonance spectrum method, etc. The most widely used in the country is the viscous measurement method and the microfiltration method. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Listen to the doctor and put your posture. Normal value (1) Viscosity detection method: 1 Rotary viscometer detection method 180s-1 < 1.00. 2 capillary viscometer detection method 3.07 ~ 3.95. (2) Microporous membrane method: 1 whole blood filtration method 0.19 ~ 0.39. 2 red blood cell suspension filtration method 0.90 ~ 1.06. Clinical significance Abnormal results: Increased red blood cell deformability decreased, blood flow decreased, see hypertension, coronary atherosclerotic heart disease v (coronary heart disease), stroke, hyperlipidemia, diabetes, pulmonary heart disease, liver disease, peripheral vascular disease, acute myocardial Infarction, shock, burns and certain blood diseases. The people who need to be examined have dizziness, fatigue, insomnia, forgetfulness, limb numbness, chest tightness, palpitations, fever and other symptoms. High results may be diseases: pediatric hereditary elliptic polycythemia, primary macroglobulinemia, renal damage considerations Before the inspection: no special contraindications. Listen to the doctor and put your posture. Inspection process Inspection methods: There are many methods for determining the deformability of red blood cells. In principle, they can be divided into two categories. One is to subject the blood samples to a large geometric scale measurement system to undergo shear stress, and to determine the average of red blood cells in the flow. Deformable properties such as viscosity measurement calculations and laser diffraction methods. Another method is to use a narrow channel system to pass red blood cells one by one, such as micropipette method and filtration method, the former can determine the deformability of a single red blood cell, and the latter reflects the deformation performance of a group of red blood cells. Not suitable for the crowd Hemophilia and diffuse intravascular coagulation. Adverse reactions and risks Infection: Do not touch the dirt after blood collection. Do not wash your hands immediately to avoid infection.