Monocyte count (MONO)

Monocyte count (MONO) is a type of white blood cell differential count that refers to the absolute count of monocytes in the blood. Monocytes have the same progenitor cells as granulocytes, and mature monocytes are released into the blood. However, in fact, monocytes are not fully mature. After staying in the blood for about 3 to 16 days, the tissues enter the tissue and continue to develop into macrophages. The macrophages in the blood and tissues constitute a mononuclear phagocytic system. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Included items: Mononuclear cell ratio (MONO%) Tips: Maintain a normal mindset. Normal value The reference value is 0.3-0.8*10^9/L for males, 0.3-0.8*10^9/L for females, and 0.3-0.8*10^9/L for newborns. Clinical significance Mononuclear cells: (1) Infected tuberculosis active or worsening period, subacute bacterial endocarditis, infectious mononucleosis, acne, chickenpox, measles, mumps, leptospirosis, scarlet fever, protozoa Diseases (malaria, kala-azar, rickettsial disease), etc. (2) Hematological diseases Chronic monocytic leukemia, Hodgkin's disease, Banti syndrome, Gaucher disease, and the like. (3) Chronic disease hepatitis, cirrhosis, etc. (4) Recovery period of other acute infectious diseases, recovery period of agranulocytosis, etc. Monocyte reduction has no important clinical significance. Precautions The classification of white blood cells is greatly changed by factors such as technical factors and cell distribution factors, so the dispersion of classification counts is large, and the proportion of neutrophils and lymphocytes, which account for a large proportion in the classification, is normally distributed, accounting for a small proportion. Such as eosinophils, basophils and monocytes are Powson distribution. According to Rümke et al., the 95% and 99% confidence limits for white blood cell differential counts. There is a 95% chance of counting white blood cells in the same blood sample or another blood sample of the same patient. The categorical count of granulocytes ranges from 53% to 67%, and the probability range of 99% is 51%. ~69%. If it exceeds this range, it is considered that the classification and counting error is too large, which does not meet the quality requirements, and should be taken seriously. Inspection process (1) Take a small drop of blood on one end of the slide, and use a push piece to push the circumference around 35 ° ~ 45 ° to leave a proper amount of voids, which can distinguish the thin blood of the head, body and tail. The length of the blood film is not less than 2.5 cm, and the remaining space to the other end of the slide is about 1 cm. The blood film is dried and stained. (2) Wright's Giemsa composite staining method: flat blood sample on the staining rack, add 3 to 5 drops of staining solution, immediately cover the blood film, add about 5 to 10 drops of buffer after about 30s, gently shake the glass The tablets or lightly blow the mixture to mix the dye solution with the buffer solution. After 5 to 10 minutes, the dye solution is washed away with water and dried for microscopic examination. (3) Rapid method: place the rapid dyeing liquid A and liquid B in the appropriate size dyeing tank, immerse the blood film in the liquid for 30s, wash it, then immerse it in the liquid for 30s, wash it, and dry it for microscopic examination. (4) Microscopic examination: Select the junction of the meninges and tails, and the red blood cells have not overlapped with oil mirrors. The examination should have a certain direction from top to bottom and left and right, and take into account the edges of both sides of the long film of the blood film, otherwise it will affect various cells. Detection rate. Count 100 to 200 white blood cells, classify them according to their morphology, and find the percentage. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Infection: Do not touch the dirt after blood collection. Do not wash your hands immediately to avoid infection.