β-Thromboglobulin

Β-thromboglobulin (β-TG) is a platelet-specific globulin that is released from the platelet alpha particles into the plasma under the stimulation of an inducer. The determination of β-TG in plasma can reflect platelet specificity. Release reaction. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Below normal, it is easy to cause blood diseases. Normal value: β-TG: 6.6-26.2ng/ml PF4: 0.9-5.5ng/ml Above normal: Elevation: found in myeloproliferative diseases, thrombocytopenia, vascular pseudohemophilia. negative: Positive: Tips: Ingest some high-fiber and fresh vegetables and fruits, balanced nutrition, including essential nutrients such as protein, sugar, fat, vitamins, trace elements and dietary fiber. Plasma β-TG was 16.4±9.8 ng/ml and PF4 was 3.2±2.3 ng/ml. Clinical significance The plasma β-TG and PF4 were measured by radioimmunoassay. Elevation: myeloproliferative diseases, thrombocytosis, von Willebrand's disease, cerebral infarction, thrombotic thrombocytopenic purpura (no increase in primary thrombocytopenic purpura), DIC, deep venous thromboembolism, diabetes, Epilepsy, migraine, oral contraceptives. The result is low, the disease may be: the result of diffuse intravascular coagulation is high. Possible diseases: precautions for essential thrombocytosis (1) The stock solution must be sucked up and washed repeatedly with the application solution several times. (2) Both should be mixed after the sample is added, and the chromogenic matrix diluent and the enzyme-labeled antibody should be freshly prepared before use. Inspection process Method of operation (1) The 96-well ELISA plastic plate provided is coated and lyophilized, so it can be directly reacted. (2) Reaction on the sample: 1 Place the ELISA coated plate flat, and measure the first and second rows as the standard. Add 7 different concentrations of standard standard 100μl from top to bottom (ie from “A” to “G”), row 8 ("H") No standard was added, and only 100 μl of sample buffer (EL-2) was added as a non-specific "hole". 2 From the third row, determine the sample to be tested, and add 100 μl of the sample dilution to each well. 3 set at room temperature (> 22 ° C) for 2 h or in a 37 ° C incubator for 1 h, and need to be covered. (3) Enzyme label reaction: 1 The first reaction plate which was allowed to stand for 3 hours, the internal liquid was aspirated, and washed 4 times with a plate washing solution (EL-1). Specifically, 200 μl of a plate washing application solution (EL-1) was added to each well. Gently shake a few times and then aspirate. The inner liquid was patted dry on absorbent paper, and this was repeated 4 times. 2 Add the diluted enzyme label application solution from left to right, from start to finish, add 100 μl to each well, and then incubate for 1 h at room temperature for 1 h or 37 ° C incubator. The 8th row ("H") well still uses the sample buffer without the enzyme labeling solution. (4) Color reaction: 1 After the enzyme label reaction for 1 hour, the application solution (EL-1) should be washed immediately with the plate, washed repeatedly 4 times, blotted dry, and then subjected to a color reaction. 2 Add 200μl of chromogenic matrix dilution solution to each well. Record the time immediately after adding. All should be completed within 4.5~5min. Pay attention to control time or color reaction speed. The color should not be too light or too deep (ie 1st) The row A value is above 1.0, and the eighth row is best at around 0.1). 3 The color reaction to be colored has been observed to have obvious gradient discoloration (reference reaction time is 4.5 to 7.0 min). Immediately in the original previous order, 50 μl of 3 mol/L H2SO4 application solution was added to the well to terminate the reaction. After 10 min, the measurement was carried out on a ELISA reagent using a 492 nm filter. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.