D-dimer (DD)

Plasma D-dimer assay is an assay to understand the function of secondary fibrinolysis. There are many influencing factors in this test, and the results must be verified when judging. Increased or positive is seen in secondary fibrinolysis, such as hypercoagulable state, disseminated intravascular coagulation, kidney disease, organ transplant rejection, thrombolytic therapy. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Tips: Keep a normal mindset. Normal value Qualitative: negative. Quantification: FDP <1 mg/L in serum; D-dimer in plasma 20-400 μl; this value is for reference only, and the specificity of each kit must be considered. Clinical significance D dimer mainly reflects fibrinolytic function. Abnormal result Increased or positive is seen in secondary fibrinolysis, such as hypercoagulable state, disseminated intravascular coagulation, kidney disease, organ transplant rejection, thrombolytic therapy. High results may be diseases: bladder tumor, glomerulonephritis precautions (1) D-dimer can identify primary fibrinolysis and secondary fibrinolysis, and primary fibrinolytic D-dimer does not increase. (2) Under normal circumstances, D-dimer determination in the elderly is higher than that in young people. Inspection process Bleeding and coagulation tests. Latex agglutination Principle: The D-dimer in the plasma to be tested interacts with the monoclonal antibody coated on the latex particles to produce a flocculent precipitation reaction. Enzyme linked immunosorbent assay (ELISA) Principle: The antigens established by two monoclonal antibodies against D-dimer were used as the center, two antibody sandwich methods were added, and the substrate of horseradish peroxidase was added for color reaction. NycoCardD-dimer assay Principle: The immunofiltering colloidal gold color reaction method adopts the same antibody sandwich, that is, the antigen in the plasma (D-dimer) is captured by the coated antibody, and the same kind of antibody coupled with colloidal gold is added for color development. Therefore, the antigen is the center, and the antibody is sandwiched on both sides, but is the same antibody. Due to the high specificity of the antibody, it can be combined with various fragments containing D-dimer to increase the sensitivity of the test. Although it can be combined with the D-segment, the hooking phenomenon does not occur. D-dimer is of great value not only in deep vein thrombosis, but also in other diseases. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.