Hepatitis B Antigen Antibody Test

Hepatitis B is caused by hepatitis B virus (HBV), which is known to have three antigens. Hepatitis B patients are prone to glucose metabolism and lipid metabolism disorders. When checking liver function, check blood sugar and blood lipids at the same time. They must not eat before the test, because some substances in the food are absorbed into the bloodstream through the digestive tract after eating, so that blood components can occur. Changes, such as blood sugar, chloride and lipids will increase; the fat content of the blood will increase temporarily after eating, so that the serum becomes white translucent or chyle, the accuracy of the test results. Under the action of glucocorticoids, the fasting blood glucose of the normal body begins to rise after 6 o'clock in the morning, and reaches the peak at 8 o'clock in the morning. At this time, the situation in the body cannot be truly reflected. Therefore, the blood test should be taken at 6 o'clock in the morning on an empty stomach. For example, in an emergency patient or a patient on a remote road, if necessary, blood can be taken after 6 hours of eating, because the blood will gradually clear out (ie, the serum is restored to its original state), but the blood lipid test should be performed 12 hours after fasting. Basic Information Specialist classification: Infectious disease examination and classification: liver function examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Hepatitis B surface antigen (HBsAg) positive: acute infection or chronic hepatitis B surface antigen carriers, have a certain infectivity. Hepatitis B surface antibody (HBsAb) positive: indicates that he has been infected with hepatitis B, or has been vaccinated with hepatitis B vaccine, and has now developed immunity. Hepatitis B e antigen (HBeAg) positive: Hepatitis B virus is replicating and infectious, and it can be an acute acute infection of hepatitis B virus. The higher the hepatitis B virus DNA level, the higher the e antigen positive level. This long-term positive in two pairs of hepatitis B suggests that hepatitis B virus continues to replicate in the body, chronic inflammation of the liver, and poor prognosis. Hepatitis B e antibody (HBeAb) is positive: Hepatitis B virus infection has entered the late stage, when the virus replication is reduced and the infection is weakened. The recovery period after chronic hepatitis B virus infection or chronic infection can be positive. Hepatitis B core antibody (HBcAb) positive: suggesting acute hepatitis B virus infection, is also a sign of hepatitis B virus replication and infectious, acute hepatitis B, chronic hepatitis B, cirrhosis, etc. can be positive. Tips: Be an empty stomach before checking. Normal value negative. Clinical significance 1. HBsAg positive indicates that the patient is in HBV infection status, which is found in the incubation period of HBV infection, acute hepatitis B, chronic or prolonged hepatitis B activity. If the liver function is normal only HBsAg positive, it may be long-term carriers of HBV or liver function has returned to normal and HBsAg has not turned negative. 2, anti-HBS positive, is an immune index for HBV, which is not only the effect after vaccination with hepatitis B vaccine, but also the performance of hepatitis B in recovery period. Anti-HBS, anti-HBC, and HBsAg are all indicators of hepatitis B infection rate, and any one is positive, indicating that they have been infected with HBV. 3, anti-HBc positive, it is not a protective antibody, but an indicator of liver cell damage caused by HBV, an early marker of acute infection, long-lasting or even life-long retention, high titer often indicates that there is in vivo HBV replication is contagious, and low titers suggest an indicator of previous infection. Anti-HBc has three types of IgG, IgA and IgM, of which anti-HB-CIgM is positive, found in the early stage of acute hepatitis B, and much more sensitive than HBsAg. In addition, chronic prolonged hepatitis active, cirrhosis and liver cancer patients as well as asymptomatic HBV carriers can also be detected. Anti-HBclgA is found in the serum of patients with chronic hepatitis, cirrhosis and liver cancer, but the positive rate of chronic hepatitis active is higher than that of chronic persistent hepatitis. Anti-HBC IgG low titers indicate previous infection, while high titers indicate that HBV is being infected. 4, HBeAg positive, is an early sign of acute HBV infection, indicating that liver cells have progressive damage and high infectivity. HBeAg is elevated before hepatitis B is aggravated, which helps predict hepatitis. Among the chronic persistent hepatitis B and HBsAg carriers, HBsAg, HBeAg and anti-HBc were positive. This "big three yang" was highly contagious and difficult to turn negative; only HBeAg positive was negative. Seeing anti-HBe positive, this "small three-yang" person generally indicates that the condition is recovering and the infection is weak. 5, anti-HBe positive, not a protective antibody, it can not inhibit the proliferation of HBV, more common in patients with HBeAg negative, meaning a manifestation of reduced transmission. In the case of HBsAg and anti-HBS negative, if anti-HBe and anti-HBc can be detected, it can be confirmed as a recent infection of hepatitis B. Positive results may be diseases: liver cirrhosis, hepatitis B precautions 1. Have an empty stomach before the examination. Hepatitis B patients are prone to glucose metabolism and lipid metabolism disorders. When checking liver function, check blood sugar and blood lipids at the same time. They must not eat before the test, because some substances in the food are absorbed into the bloodstream through the digestive tract after eating, so that blood components can occur. Changes, such as blood sugar, chloride and lipids, will increase, and the fat content of the blood will increase temporarily after eating, so that the serum becomes white translucent or chyle, and the accuracy of the test results. Under the action of glucocorticoids, the fasting blood glucose of the normal body begins to rise after 6 o'clock in the morning, and reaches the peak at 8 o'clock in the morning. At this time, the situation in the body cannot be truly reflected. Therefore, the blood test should be taken at 6 o'clock in the morning on an empty stomach. For example, in an emergency patient or a patient on a remote road, if necessary, blood can be taken after 6 hours of eating, because the blood will gradually clear out (ie, the serum is restored to its original state), but the blood lipid test should be performed 12 hours after fasting. 2. Forbidden to take certain drugs. Taking medication may affect the accuracy of liver function tests. Such as: taking vitamin D, adipine, chlortetracycline, nitrofurazone and other drugs will affect the determination of jaundice index: taking opioids can lead to increased transaminase activity; with antipyrine, sulfonamides, Drugs such as aminosalicylic acid (PAS), procaine, and urotropine can block the determination of urinary bile cell response. The use of PAS can increase the phenolic quaternary tetrabromide iodate retention test (BSP). 3. Fasting certain foods. The day before the patient's liver function is checked, it is best not to eat foods rich in carotene and lutein, because these foods will make the serum yellow, affecting the determination of the jaundice index; high-fat foods such as fritters, milk, chocolate, Egg meat products, etc., can significantly increase the blood finger test, so it is necessary to ban the consumption of finger foods 10 hours before the blood draw. Inspection process 1. Experimental preparation: The kit was taken out from the refrigerated environment, equilibrated at room temperature for 30 min, and the concentrated washing solution was diluted 1:20. 2. Add 0.05 ml per well to the test specimen, and set 2 wells of anti-HBc positive control and anti-HBc negative control, and 1 well of blank control. The 11:30 dilution serum (plasma) test results represent clinical significance. 2 The original serum (plasma) test results represent epidemiological significance. 3, add enzyme conjugate 0.05ml per well, blank control is not added, fully mixed, incubated at 37 ° C for 30min. 4, the washing machine wash plate select washing process 5 times to wash the plate, and finally patted dry. 5, add coloring agent first add coloring agent A, 0.05ml per well; add coloring agent B, 0.05ml per well; mix thoroughly, place at 37 ° C for 15 min in the dark. 6. Stop the reaction and add 0.05 ml of stop solution to each well and mix. 7. Measure the reading with the microplate reader, and select the single wavelength of 450nm (with blank hole calibration) or dual wavelength 450/630, and read the OD value of each hole. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.