iron staining

In the bone marrow granules, hemosiderin is called extracellular iron; the cells also contain iron, which is called intracellular iron. The ferric ion is not firmly bound to the protein in the molecule. After treatment, it is free and can be used in acidic potassium ferrocyanide solution. The Prussian blue reaction is produced and stained. Basic Information Specialist classification: growth and development check classification: microscopy Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Reduced in iron deficiency anemia. Normal value: Iron granules: 19-44 Above normal: Elevated in iron granule anemia, myelodysplastic syndrome (iron granule refractory anemia) and so on. negative: Positive: Tips: Slides must be clean and non-staining, and need to be iron-free. Normal value The extracellular iron is "+" to "++", the intracellular iron counts 100 young red blood cells, and the iron granules are 19 to 44. Clinical significance Reduced in iron deficiency anemia. In the case of iron deficiency anemia, the extracellular iron in the bone marrow is significantly reduced or even disappeared, and the iron granule cells are decreased. After treatment with iron, extracellular iron increased. Iron particle staining can be used as a method to diagnose iron deficiency anemia and guide iron therapy. Elevated in iron granule anemia, myelodysplastic syndrome (iron granule refractory anemia) and so on. In addition, it can also be seen in hemolytic anemia, nutritional megaloblastic anemia, aplastic anemia and leukemia, infection, cirrhosis, chronic nephritis, uremia, multiple blood transfusions, hemochromatosis, etc., extracellular iron can also increase. Low results may be diseases: paroxysmal nocturnal hemoglobinuria in children, iron deficiency anemia in the elderly, hereditary hemochromatosis, high iron deficiency anemia may be a disease: pediatric renal anemia, pediatric secondary thrombocytosis , postpartum hemorrhage, chronic disease anemia in the elderly, iron utilization anemia, globin-forming anemia, globin-forming anemia retinopathy, anemia caused by malignant tumors (1) The slide must be clean and free of dirt and must be treated with no iron. Treatment method: the new film was soaked in the cleaning solution for 24 hours, rinsed and immersed in 95% ethanol for 24 hours, dried and then immersed in 5% HCl for 24 hours. The distilled glass was repeatedly dipped and washed, and taken out for drying. (2) Acidic potassium ferrocyanide must be used in the presence of acid. (3) It is best to use the stain of the smear of the marrow to observe the extracellular iron, because this smear contains more bone marrow particles. (4) The intracellular iron count should be based on medium and late red blood cells. Inspection process (1) Add 1 part of concentrated hydrochloric acid to 5 parts of 200 g/L potassium ferrocyanide, freshly prepare, and filter for use. (2) The bone marrow pieces were fixed with methanol for 10 min. (3) Add the dye solution prepared above for 30 minutes, rinse with tap water. If the room temperature is too low, the dyeing time can be prolonged. (4) Counterstaining with 5 g/L of Sahuang aqueous solution for 1 min. Washed, dried, and examined. Not suitable for the crowd Patients with hematopoietic dysfunction, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is necessary, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.