Helicobacter pylori detection

Helicobacter pylori examination, common gastric mucosal tissue biopsy for rapid urease test, Gram stain microscopy and 10% CO2 separation culture. Helicobacter pylori is a unipolar, multi-flagellate, blunt-ended, spiral-curved bacterium. It is 2.5 to 4.0 μm long and 0.5 to 1.0 μm wide. On the surface of gastric mucosal epithelial cells, the surface is usually spiral or curved. When grown on a solid medium, in addition to the typical morphology, rods or spheres may sometimes appear. Helicobacter pylori is a microaerobic bacterium, which requires 5 to 8% of ambient oxygen and cannot grow in the atmosphere or in an absolute anaerobic environment. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Not infected with Helicobacter pylori. Positive: Positive indicates gastric and duodenal digestive tract inflammation and ulcers. Tips: Check the dinner the day before to eat less slag diet, rest at night. Normal value Helicobacter pylori was not detected. Clinical significance If the culture is positive, it can be diagnosed. According to research, the bacteria are related to the following diseases. 1. The positive rates of positive isolation in peptic ulcer, gastric and duodenal mucosa were 57%-85% and 86%-96%, respectively. 2. The positive rate of gastric mucosal isolation and culture of chronic gastritis was 85%. 3. The positive rate of isolation and culture of duodenitis is high, and can be as high as 100% in the active period. Positive results may be diseases: Helicobacter pylori infection in children, chronic gastritis in children, recurrent abdominal pain in children, erosive gastritis 1, check the day before dinner to eat less slag diet, rest at night. 2, check the fasting on the day (do not eat breakfast, do not drink water, do not take medicine), do not smoke. 3. Perform pharyngeal anesthesia (gastroscope glue) 5 to 15 minutes before the examination to relieve discomfort. 4. Don't be nervous during the examination. Cooperate closely with the doctor. Obey the instructions of the medical staff during the examination and slowly take a deep breath to reduce the uncomfortable reaction and reduce the inspection time. 5. After the inspection, keep quiet for 30 minutes to 1 hour. 6. Fasting 30 minutes after the examination. 7, after the swallowing action resumes freely for 2 hours, then enter the diet to avoid food inhalation into the lungs. 8. Fasting irritating food on the day of biopsy. Inspection process The indirect ELISA method was used for serological detection. The bacteria solution was determined by ultrasonic pulverization, centrifugation, protein quantification, and the same amount was mixed as an antigen. The serum of the patient to be tested was used as a primary antibody, and the enzyme-labeled goat anti-human IgG was used as a secondary antibody, and o-phenylenediamine was used as a substrate for color development, and the result was judged according to the optical density. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.