Sudan Black B stain

SBB staining is more sensitive than POX in identifying leukemia types, especially in identifying acute non-lymphocyte leukocytes and acute lymphocyte leukocytes. Sudan black is a fat-soluble dye that stains lipids such as neutral fat, phospholipids and cholesterol in cells into brown-black particles and localizes to the cytoplasm. Basic Information Specialist Category: Inspection Category: Microscopy Applicable gender: whether men and women apply fasting: not fasting Tips: Please relax when checking. Normal value The positive product is a black or blue-black particle located in the cytosol. Clinical significance Similar to POX staining, but more sensitive than POX staining. The value of identification of acute leukemia is greater. Positive results may be diseases: malignant histiocytosis, leukemia, aplastic anemia considerations Hodgkin's disease, pernicious anemia, Gaucher disease showed positive or weak positive reaction; when aplastic anemia, positive increased. Inspection process (1) The dried smear was immersed in 10% formaldehyde saline for 10 min. (2) Rinse with tap water for 10 min, rinse with distilled water, and dry. (3) Place in the dye solution and stain at 37 ° C for 1 to 12 hours. (4) Decolorize in a 70% ethanol solution for 3 min and dry. (5) Counterstaining with Wright's dye solution for 20 to 30 minutes. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.