Bone marrow lymphocyte system

The bone marrow lymphocyte system is a type of bone marrow cytology. Bone marrow cytology is the most valuable for diagnosing hematopoietic diseases. It is also useful for diagnosing other non-hematopoietic diseases, hepatosplenomegaly with unexplained fever, cachexia, and unexplained causes. Differential diagnosis. Basic Information Specialist classification: Oncology examination classification: microscopy Applicable gender: whether men and women apply fasting: not fasting Tips: hemophilia and diffuse intravascular coagulation, if there is no special need, do not do bone marrow puncture examination. Normal value The original lymphocytes are 0 to 0.004 (0 to 0.4%). Young lymphocytes range from 0 to 0.021 (0 to 2.1%). Lymphocytes range from 0.107 to 0.431 (10.7% to 43.1%). Clinical significance Increased: acute lymphoblastic leukemia, chronic lymphocytic leukemia, infectious mononucleosis, infectious lymphocytosis, whooping cough, etc. Acute lymphocytic leukemia is mainly caused by proliferative lymphocytes and proliferating lymphocytes. In other cases, mature lymphocytes are mainly increased, but the degree and cell morphology are different. Chronic lymphocytic leukemia is significantly increased by lymphocytes (more than 50%). Lymphocytosis of the above infectious diseases is not significant. High results may be disease: mediastinal non-Hodgkin's malignant lymphoma, mediastinal non-Hodgkin's lymphoma, pediatric autoimmune lymphocytosis syndrome, pediatric Viscot-Aldrich syndrome, peripheral lymph node tuberculosis in children Precautions Preoperative preparation: The patient is placed in accordance with the doctor's instructions. Inspection process Inspection method: bone marrow examination. Inspection process: 1. Select the puncture site. 2. Anesthesia. 3. Fix the length of the needle. 4. The doctor's left thumb and finger are fixed at the puncture site. The right hand-held bone marrow puncture needle is inserted perpendicularly to the bone surface. If the sternum is puncture, it should be inserted at an angle of 30o to 40o with the bone surface. When the needle tip touches the bone, rotate the needle along the long axis of the needle of the needle and push it forward to slowly penetrate the bone. 5. Extract the bone marrow fluid and pull out the needle core, connect the dry syringe (10m1 or 201m1), and use the appropriate force to extract the bone marrow fluid. Bone marrow cytology check steps: 1. Smear: It is required that the smear slide and push piece should be clean, no putty pollution, the smear should be thin and uniform, the number of smears is about 10, and two blood samples are used for comparison. 2. Dyeing: commonly used Wright-Gemsa mixed staining; cytochemical staining is often used together. 3. Low magnification examination: to determine the degree of bone marrow hyperplasia, usually the ratio of mature red blood cells to nucleated cells in bone marrow slices to determine the bone marrow hyperplasia 4. Oil Mirror Inspection: Select the cells to be evenly distributed. Under the oil microscope, classify and count at least 200 nucleated cells, and pay attention to whether there is qualitative change. Not suitable for the crowd Hemophilia and disseminated intravascular coagulation, if there is no special need, do not do bone marrow puncture. Adverse reactions and risks May cause bleeding and infection.