phi(φ) body staining

The φ body in the cytoplasm of leukemia cells is treated with 3,3'-diaminobenzidine (DAB), a hydrogen peroxide base solution, and a nitrite-treated catalase dye to catalyze the oxidation of DAB to a blue precipitate. Positioned within the cytoplasm. Basic Information Specialist classification: cardiovascular examination classification: microscopy Applicable gender: whether men and women apply fasting: not fasting Tips: Please relax when checking. Normal value Under the light microscope, the φ small body has a brown thin rod shape or a spindle shape, and a large round particle shape, one or more. Clinical significance It is mainly found in primary and young cells of patients with acute myeloid leukemia (rapid granules, acute promyelocytes, acute monocytic leukemia), and in protoplasts and young cells of some "myeloid" blasters of chronic granules. Precautions Φ small body staining has a strong pathological significance and contributes to the identification of leukemia types. Inspection process (1) DAB staining step: The dried bone marrow (blood) tablets were fixed with glutaraldehyde-formaldehyde fixative for 2 min, rinsed with physiological saline and stored in saline until staining. Incubate for 3 min in matrix solution, rapidly rinse 3 times with 0.05 mol/LTris-HCl (pH 7.3-7.6), set 5.0 g/L, 2 min in copper nitrate solution, rinse with normal saline, directly microscopically or counterstain after drying. After the microscopic examination. (2) Improved pasteurization step: After the 1DAB staining is completed, the specimen is still stored in physiological saline until the next step of staining begins. 2 Dyeed in hematoxylin dyeing solution for 1 min, taken out and washed with water. 3 Immerse in dilute hydrochloric acid ethanol solution for 0.5 min and wash with water for 2 min. 4 diluted lithium carbonate for 1 min, washed with water for 2 min. 5 Dehydrated in 50%, 70%, 80%, 95% ethanol for 0.5 min. 6 dyed in orange G6 for 1 min. Dip twice in 795% ethanol for 10 s each time. 8 stained in EA50 solution for 10 min. Dip twice in 995% ethanol for 3 min each time. 10 immersed in absolute ethanol for 2 times each time for 1 min. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.