Plasma protamine paracoagulation test (3p test)

The plasma protamine coagulation test, also known as the 3P test, is an older test for detecting fibrin degradation products. The protamine sulfate can dissociate the fibrin monomer in the soluble complex of the fibrin monomer and the fibrin degradation product, and the fibrin degradation product is self-polymerized to be visible in the form of fibrous, floc or jelly. That is to say, the existence of fibrin degradation products is reflected, and different tests can be obtained according to the type of fibrinolysis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative is seen in normal subjects, advanced disseminated intravascular coagulation, and primary fibrinolysis. Positive: Positive in the early or middle stage of disseminated intravascular coagulation, thrombotic disease, thrombolytic treatment, hypercoagulable state. Tips: Specimens after blood collection to avoid hemolysis. Normal value negative. Clinical significance The plasma protamine coagulation test is positive in the early or middle stage of disseminated intravascular coagulation, thrombotic disease, thrombolytic therapy, and hypercoagulable state. False positives should be excluded. The plasma protamine paracoagulation test was found to be negative in normal subjects, advanced disseminated intravascular coagulation, and primary fibrinolysis. Positive results may be disease: neonatal disseminated intravascular coagulation results may be negative disease: shock, hemangioma thrombocytopenia syndrome considerations (1) Sodium oxalate, heparin or disodium edetate cannot be used as an anticoagulant. (2) There are false positive results, such as specimens placed in the refrigerator, blood is not smooth, anticoagulation is uneven, local bleeding (hematemesis, hemoptysis, major surgery, trauma). (3) There are false negative results, such as when the temperature of the water bath is too low and reheated to 37 ° C, when the fibrinogen content is too low. Inspection process (1) Take 0.5 ml of platelet-poor sodium anticoagulant plasma. (2) Place a 37 ° C water bath for 3 min. (3) Add 10 ml of 10 g/L protamine sulfate solution, mix well, set a 37 ° C water bath for 15 min, and observe the results immediately. Not suitable for the crowd Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the test, and patients with a history of drug administration in the near future are prohibited. Adverse reactions and risks 1, dizziness or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 2. Risk of infection: If you use an unclean needle, you may be at risk of infection.