hepatitis B core antigen

Hepatitis B core antigen (HBcAg) is a single polypeptide with a molecular weight of 18848u. HBcAg plays an important role in HBV infection. It can reflect the presence of Dane particles in serum and HBV replication in the liver, and can complement and complement each other with other HBV serological markers. It is difficult to detect free HBcAg in serum simply because its antibody has a strong affinity and can rapidly bind to HBcAg in serum to form an immune complex. HBcAg was detected by ELISA and IRMA double antibody sandwich method. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The appearance of HBcAg antigen can be used as a marker for infectious disease of hepatitis B. Tips: Check the specimen, take 2ml of venous blood, and separate the serum for measurement. Normal value negative. Clinical significance HBcAg is present in high plasma and liver tissues and is mainly found in Dane particles, which are not detected in blood. Positive results may be diseases: precautions for hepatitis B antigenemia The appearance of HBcAg antigen can be used as a marker for infectious disease of hepatitis B, and it can help to determine the condition and prognosis of hepatitis B. Inspection process After the serum to be tested is treated, it is basically determined by the same as HBsAg. Treatment of serum to be tested: Take 0.4ml of serum, add 1.0ml of normal saline, mix and centrifuge at 4000r/min for 20min, discard the supernatant, add anti-HBc50μl, incubate for 1h at 37°C, displace 4°C overnight, remove the next day. After centrifugation at 3 ml of NaCl, 4000 r/min for 30 min, the supernatant was discarded, and the HBV immune complex was obtained, and then washed twice with NaCl for the purpose of removing the interference of the remaining anti-HBc. Extracting HBcAg to cleave HBV particles, adding 4% NP-40100μl to the precipitate, and pulverizing the precipitate with a glass rod, and incubating at 37 ° C for 15 min, which is the treated serum to be tested. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.