HBV anti-pre-S2 antibody

The pre-S2 antigen in the serum of patients with acute hepatitis B induces the pre-S2 antibody (Anti-PreS2) before viral clearance, but there is no such special conversion in the process of becoming chronic, suggesting that this antibody may predict the recovery of HBV infection, and resistance Like HBe, it is a marker of reduced viral replication levels. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: It is suggested that patients with acute hepatitis B have a good prognosis. Tips: This test requires an empty stomach for blood draw. Normal value negative. Clinical significance Anti-PreS2 is a neutralizing antibody against HBV, which has the effect of clearing the virus. The early appearance of anti-PreS2 in serum suggests that the prognosis of patients with acute hepatitis B is good. Positive results may be diseases: hepatitis B, hepatitis B virus precautions Dynamic observation of the pre-S2 antibody can predict the outcome of the disease. Inspection process 1, according to the material: blood. 2. Principle of determination of pre-S2 antigen of hepatitis B virus: Double antibody sandwich method: The microporous reaction plate was coated with monoclonal PreS2 antibody, PreS2 was reacted with the serum to be tested, and HRP-labeled anti-HBs were added to form a sandwich, and finally the substrate was colored. 3. Reagents: There are a complete set of kits available in China. Including: monoclonal anti-PreS2 antibody; HRP-anti-HBs; horseradish peroxidase and horse anti-HBs-IgG or monoclonal anti-HBs, a combination prepared by the modified sodium periodate method. 4. Operation method: Follow the kit instructions or follow the procedure: Add appropriately diluted monoclonal anti-PreS2 to the wells of the polystyrene microplate, 100 μl per well, overnight at 4 °C. Wash 3 times with distilled water. Add 100 μl of serum to be tested and set positive and negative control wells. Wash at 37 ° C for 2 h. 100 μl of diluted HRP anti-HBs was added, washed at 37 ° C for 2 h, 5 times. Add 100 μl of o-phenylenediamine-H 2 O 2 substrate solution at 37 ° C for 20 min. The reaction was terminated by adding 2 mol/L H 2 SO 450 μl. The absorbance was measured at 492 nm using an enzyme-linked detector, and P/N ≥ 2.1 was positive. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.