Hepatitis B Surface Antigen

Hepatitis B surface antigen (HBsAg) is the outer shell of hepatitis B virus (HBV), which is present in the blood, body fluids and secretions of infected persons. It is generally present in combination with HBV and is an important serological marker for judging HBV infection. One. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normally negative. Positive: Serum HBsAg positive is mainly seen in: (1) latent state after HBV infection. (2) Acute phase of hepatitis B virus. (3) Chronic hepatitis (prolonged or active hepatitis) cirrhosis or liver cancer after hepatitis. (4) HBsAg carriers. Tips: You must take a blood test on an empty stomach. Do not eat for 8-12 hours before blood draw, but drink a small amount of water. Normal value Normally negative. Clinical significance The normal population of HBsAg in China is 10%, and serum HBsAg positive is mainly found in: (1) Latent status after HBV infection. (2) Acute phase of hepatitis B virus. (3) Chronic hepatitis (prolonged or active hepatitis) cirrhosis or liver cancer after hepatitis. (4) HBsAg carriers. Positive results may be diseases: hepatitis B, chronic hepatitis precautions (1) Shake the reagent before use, and discard 1 to 2 drops and then add it vertically. Pay attention to even force. (2) The kit taken out from the refrigerated environment should be equilibrated at room temperature for 30 minutes before testing. The rest should be sealed in time and stored in the refrigerator for later use. (3) The specimen to be inspected for refrigeration should be equilibrated at room temperature for 30 minutes and then tested. (4) NaN3 antiseptic is not available for the specimen to be inspected. Inspection process Take the coated solid phase carrier reaction strips and place them on the frame and number them. 50 μl of serum to be tested was added to each well, and each plate was positive, negative and blank. Then, an enzyme-labeled anti-HBs1 drop was added to each well, and the micro-oscillator was shaken for 1 to 2 min, and placed in a 37 ° C water bath for 30 min. Remove the reaction plate, remove the liquid in the well, wash it with the washing liquid 5 times, each time to stand for 1 min, add the substrate solution (50 μl of liquid A and liquid B) after air drying, and develop color at 37 ° C for 10 min in the dark, with 2 mol. The /LH2SO41 drop terminates the reaction. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally no complications and harm.