Acantholysis sign test

The acanthosis cell release test is one of the commonly used physical examination methods in dermatology, and is used to examine the position of the blister and bullae in the epidermis or under the epidermis. Basic Information Specialist classification: skin examination classification: physical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: Some bullous skin diseases such as pemphigus, bullous epidermis, and atrophic drug eruption can be positive. Tips: Prohibition before eating can cause drugs that hinder proper diagnosis, and maintain skin hygiene to reduce infection. Normal value Negative, asymptomatic. Clinical significance Some bullous skin diseases such as pemphigus, bullous epidermis, and atrophic drug eruption can be positive. Abnormal results bullous epidermis loosening atrophic drug eruption, Staphylococcus aureus scalded skin syndrome, pemphigus, bullous epidermolysis. People who need to be examined: people with suspected symptoms. Positive results may be diseases: blistering, scalp pustular erosive skin disease, drug rash considerations Inappropriate crowd: not found yet. Contraindications before the test: Prohibition of eating may cause drugs that hinder proper diagnosis, and maintain skin hygiene to reduce infection. Requirements for examination: If diagnosed, you should actively cooperate with the doctor. Inspection process 1. Clinical damage characteristics Clinically, only a red wound or erosion surface is often seen. If the probe can be inserted under the epithelium or under the adjacent mucosa without resistance, the Nissl's sign is positive, or the skin test is positive, which is helpful for diagnosis, but not big. The peeling test is used in scope to avoid increasing the suffering of the patient. The general condition of the patient is characterized by decreased constitution and even sub-quality, which is also helpful for diagnosis. In addition, the following methods are helpful for proper diagnosis. 2, cytological examination After local disinfection, the early fresh bulls are cut off to the blister top, the blister tissue is lightly scraped, applied to the slide, dried and stained with Giemsa or red hematoxylin-eosin. The typical acantholytic cell is disintegrated. . The nucleus is large and round, with deep staining and less cytoplasm. It is also known as pemphigus cells or acantholytic cells. The amount of such cells is related to the severity of the disease. 3, biopsy Near the lesion, use a mouthpiece to press the blister, then cut the epithelium and the tissue below it. 4, immunological examination (1) Immunohistochemistry: Immunofluorescence direct method shows antibodies against cell adhesion substances between layers of spine cells. (2) Detection of serum antibody substances: The indirect method of immunofluorescence is to detect circulating anti-basal cells in the cytoplasm of the patient's serum, the interstitial cells of the spinous cell layer and circulating antibodies in the spine cells. The general antibody titer is 1: 50 o'clock means that. Not suitable for the crowd May cause an infection. Adverse reactions and risks Generally no adverse reactions.