Schistosoma ELISA

In the immunological diagnosis of schistosomiasis, almost all kinds of immunological tests have been tried. In addition, there are cercaria membrane test and ring egg sedimentation test using Schistosoma cercariae and eggs as antigens. The schistosomiasis enzyme-linked immunosorbent assay is now introduced. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: A positive indication may be a schistosome infection. Tips: This method is highly sensitive and can be tested in a wide range. It has been tested for the diagnosis of various parasitic diseases. Normal value negative. Clinical significance The detection rate of fecal test eggs positive was 97% to 100%, and the normal human false positive rate was 2% to 3%. There is no obvious cross-reaction with common parasites. When this method was used to detect patients with Schistosoma mansoni, it was found to have a significant cross-reaction with Trichinella. Positive results may be diseases: schistosomiasis, pulmonary schistosomiasis, cercariae cercaria dermatitis, vaginal schistosomiasis precautions This method is highly sensitive and can be tested in a wide range and has been tested for the diagnosis of various parasitic diseases. Inspection process In the same indirect ELISA, in principle, a 1% dilution of 1% Schistosoma japonicum soluble egg antigen was coated with polystyrene plates, 200 μl per well, placed at 4 ° C overnight, washed, and then added with Tween-PBS for 1: 200 diluted serum samples, 200 μl per well of negative and positive reference serum. Each specimen should be at least duplicated. The control group was not added with serum, only PBS Tween was added, and after incubation, the appropriately diluted enzyme-labeled anti-human IgG was added, 200 μl per well. After the heat preservation and washing, the color of the A492 was read by an enzyme-based colorimeter after coloring according to the conventional substrate. The zero point was corrected with a mixture of 4 parts of o-phenylenediamine (OPD) substrate and 1 part of 2 mol/L of sulfuric acid (corrected A value greater than or equal to 0.5 is a positive reaction). Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.