schistosomiasis indirect hemagglutination test

In the immunological diagnosis of schistosomiasis, almost all kinds of immunological tests have been tried. In addition, there are cercaria membrane test and ring egg sedimentation test using Schistosoma cercariae and eggs as antigens. Indirect hemagglutination test of schistosomiasis is now introduced. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: A positive indication may be a schistosome infection. Tips: The equipment must be very clean to avoid false positives or false negatives. Normal value negative. Clinical significance The sensitivity of this method is about 93%, and the false positive rate is about 2%. It can cross-react with the serum of patients with paragonimiasis. The patient's negative conversion is slower after treatment. Positive results may be diseases: pulmonary schistosomiasis, schistosomiasis precautions (1) The equipment must be very clean to avoid false positives or false negatives. (2) It has high sensitivity and is easy to operate. Inspection process 1, according to the material: blood. 2, the principle of indirect hemagglutination test of schistosomiasis: sheep red blood cells sensitized with soluble schistosome egg antigen and corresponding antibodies in the patient's serum can exhibit agglutination reaction. 3. Reagents: There is a corresponding commercialized sensitized red blood cell reagent supply in China. 4. Operation method: strictly follow the instructions of the kit. (1) Add 4 drops (25μl/drop) of normal saline to the second hole of the first row of U-shaped micro-coagulation plate with a micro-dropper, the third hole is blank, and the fourth hole is added with 1 drop. (2) Add the serum to be tested in the first well, mix well, and take 1 drop of serum from the second well, mix well and then aspirate 2 drops, add 1 drop to each of the third and fourth holes, in the fourth After the wells were mixed, 1 drop was discarded, and the third and fourth wells were diluted 1:5 and 1:10. (3) The lyophilized sensitized red blood cell reagent is diluted with distilled water according to the instructions, and the sensitized red blood cell suspension is aspirated by a quantitative pipette, and one drop (25 μl) is added to each of the third hole and the fourth hole, and immediately shaken for 2 min at room temperature. Allow to stand for about 1 h and observe the results. (4) The interpretation criteria were the same as the general indirect hemagglutination test, and the positive reaction was found to be schistosomiasis with a serum greater than or equal to 1:10 dilution. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.