Adenovirus antibodies

The adenovirus is a icosahedral stereosymmetric, non-encapsulated, double-stranded DNA virus. The viral capsid is composed of 252 shell particles, of which 240 are hexon and 12 pentons. The hexon is distributed on 20 triangular faces and edges, and the five neighbors are located on the apex angle of the icosahedron. Each of the five adjacent bodies consists of a base and a fiber with a small knot at the top of the fiber. The length of the fibers varies, and the segmental proteins determine the characteristics of hemagglutination, and the hemagglutination-inhibiting antibodies can be produced in the body. Adenovirus can cause a variety of diseases, the most common are infantile acute respiratory infection, adenoviral pneumonia, epidemic keratoconjunctivitis, pharyngeal conjunctivitis, other diseases such as infantile diarrhea, hemorrhagic cystitis, myocarditis, meninges Inflammation and so on. Serological tests are important for determining adenovirus infection. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Prompt for acute respiratory infections, acute conjunctivitis, gastroenteritis, genitourinary infections. Tips: Check the antibody for duplicate serum samples. The first serum sample should be taken immediately after the symptoms appear. The second specimen should be taken 2 to 3 weeks after the onset of the disease. The time between taking the specimen and the test should be shortened as much as possible. If the time is long, it should be stored in a -20 °C refrigerator. Normal value negative. Clinical significance Positive acute respiratory infection, acute conjunctivitis, gastroenteritis, genitourinary infection. Positive results may be diseases: adenovirus infection, adenoviral pneumonia, myocarditis considerations Adenovirus infection specimens include throat swab specimens, nasal washes, corneal swab specimens, anal swab specimens, urine specimens, and stool specimens. The test antibody requires two serum samples. The first serum sample should be taken immediately after the symptoms appear. The second sample should be taken 2 to 3 weeks after the onset of the disease. The time between taking the specimen and the test should be shortened as much as possible. If the time is long, it should be stored in a -20 °C refrigerator. Inspection process (1) Direct method: A fluorescently labeled antibody directly reacts with a viral antigen. This method is simple, rapid, and highly specific, but has poor sensitivity. 1 Immobilization: The virus antigen was fixed on a glass slide, fixed in cold acetone for 10 min, and used as a positive and negative control. 2 Washing: Wash PBS solution of pH 7.4 3 times. 3 staining: the corresponding fluorescent antibody was added dropwise, and placed in a wet box at 37 ° C for 30 min. 4 Washing: Wash 3 times with pH 7.4 PBS solution. 5 dry: cover observation. The slide was filled with 80% glycerol and 10% 0.01 mol/L PBS pH 7.4. (2) Indirect method: The viral antigen is first bound to the first antibody (patient-suppressed serum or virus-immunized animal serum) and then reacted with a second antibody (fluorescent antibody). The law is highly sensitive. 1 Fixation: The virus antigen sample to be tested was dropped on the slide at a certain dilution, and the positive and negative controls were simultaneously fixed in cold acetone for 10 min. 2 Washing: Wash 3 times with pH 7.4 PBS. 3 Add a certain dilution of the first antibody, and apply it at 37 ° C for 30 min in a wet box. 4 Washing: Wash 3 times with pH 7.4 PBS. 5 plus a second antibody (fluorescent antibody), which was applied at 37 ° C for 30 min in a wet box. 6 Washing: Wash 3 times with pH 7.4 PBS. 7 dry, seal, and observe. (3) Determination of results: Fluorescence microscopy, according to the specific fluorescence intensity determination results, with plus or minus signs: (-): No fluorescence. (±): Very weak suspicious fluorescence. (+): Fluorescence is weak, but clearly visible. (++): Fluorescent is bright. (+++~++++): Fluorescent and wide range. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.