Serum gamma-glutamyl transpeptidase isoenzyme (iso-r-GTP)

The polyacrylamide method can classify gamma glutamyltransferase into four types, namely, γGT1, γGT2, γGT3, and γG4. Determination of gamma glutamyltransferase isoenzymes contributes to the diagnosis of liver cancer. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Before the examination, the diet was light and alcohol was banned. Check for an empty stomach in the morning. Normal value 1. Polyacrylamide gel electrophoresis (PAGE) is divided into 13 zones: I, I', II, II', III, IV, V, VI, VIIa, VIIb, VIIIa, VIIIb, VIIIc. There are only 10 zones in normal, and there are no I', II, II' belts. 2, vinegar fiber membrane electrophoresis method is divided into 4 zones: r-GTP1, r-GTP2, r-GTP3, r-GTP4. Normally, r-GTP3 is dominant. Vigor size r-GTP3>r-GTP2>r-GTP1. γGT10.6321±0.0486. γGT20.0724±0.0223. γGT30.1841±0.0314. γGT40.1114±0.0272. (γGT1>γGT3>γGT4>γGT2) (Note the specific reference value depends on each laboratory.) Clinical significance Abnormal result 1, the emergence of r-GTPII zone seen in primary and secondary liver cancer. The r-GTPI', II/II' zone is a specific new band of liver cancer, and other hepatobiliary diseases (metastasis cirrhosis, alcoholic hepatitis, cholangiocarcinoma) do not appear in the new zone. 2. The damage of hepatic parenchyma cells is mainly caused by the increase of r-GTP1. The extrahepatic biliary tract disease is mainly r-GTP2, and the r-GTP4 region can also occur, and r-GTP2>r-GTP1>r-GTP3. High results may be diseases: liver cancer, primary liver cancer, cirrhosis, hepatitis, alcoholic cirrhosis Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. Fasting for 12 hours before taking blood, taking fresh blood for inspection. When checking: When you draw blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. After inspection: 1, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 2. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Colorimetric method: 1. The type of buffer is different, and the optimum pH of the enzyme reaction is also different. Tris buffer is most suitable. 2. The free α-naphthylamine in the substrate buffer is unstable and has an inhibitory effect on the enzyme. Therefore, attention should be paid to the influence of temperature during preparation. If the absorbance of the reagent blank tube is >0.08, L-γ-glutamyl-α-naphthylamine should be recrystallized to remove free α-naphthylamine. 3, 1g / L sodium nitrite solution is unstable, should be formulated once a week. 4, the specimen is easy to use serum, and EDTA-Na2 (1mg / ml) anticoagulant plasma, other anticoagulants can affect the results. 5, GGT is relatively stable, placed at room temperature for 2 days, there is no change in enzyme activity at 4 °C for 1 week, but all inactivation at 65 °C for 1 min. Inspection process The blood of the subject is taken immediately and examined by immunological methods. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.