sugar chain antigen 50

The sugar chain antigen 50 is also called a carbohydrate antigen 50 and a cancer antigen 50, which is a glycoprotein mainly composed of sialic acid and sialic acid glycoprotein, and is also a tumor antigen. Glycolipids or glycoproteins on the surface of normal cells play an important role in the transmission, growth and differentiation of cellular information. When the cell is malignant, a tumor marker is produced due to loss of activity of the glycosyltransferase or reactivation of the invertase in the embryonic stage resulting in a change in the surface sugar structure of the cell and a change in antigenic properties. Sugar chain antigen 50 is a non-specific broad-spectrum tumor marker with a certain cross-antigenicity with sugar chain antigen 19-9. It is mainly used for the auxiliary diagnosis of pancreatic cancer, colon cancer, rectal cancer and gastric cancer. The increase is most obvious. Basic Information Specialist classification: Oncology examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Patients with pancreatic cancer have the most obvious increase. Normal value 0 to 20kU/L. Clinical significance Increased in pancreatic cancer (positive rate of 87%), colon cancer, rectal cancer, stomach cancer, lung cancer, liver cancer, gallbladder cancer, cholangiocarcinoma, ovarian cancer, breast cancer and other malignant tumors. In addition, ulcerative colitis, cirrhosis, melanoma, lymphoma, autoimmune diseases, and the like can also be elevated. High results may be diseases: pancreatic cancer, gallbladder cancer, cholangiocarcinoma, primary liver cancer, lung cancer, colon cancer, stomach cancer, rectal cancer, pleural effusion and pleurisy in the elderly, ovarian cancer 1. The positive rate of pancreatic cancer, biliary tract cancer and gallbladder cancer in malignant tumors is above 80%, the positive rate of primary liver cancer is 72%, the positive rate of ovarian cancer is 50%, the positive rate of colorectal cancer is 27%, breast cancer, The positive rate of uterine cancer and liver cancer is 20%. 2. The total reaction volume of the assay system is 250 μl/tube. In order to ensure that the antibody coated beads can always be infiltrated by the reaction solution during the incubation, the conditions of the beads washing after the incubation are the same (with a special bead washer) and easy to The beads are transferred to the corresponding measuring tubes, so a disc type reaction disk should be used. Inspection process 1. Tube number: 1 to 2 tubes are total radioactivity (T) tubes, 3 to 14 tubes are standard curve tubes, and 15 tubes are used to control serum and samples to be tested. Two sets of test tubes should be prepared, one for the sample addition reaction (a disc type plastic reaction plate can also be used). 2. Each reaction tube (except T tube) was added with C-50 antibody coated beads, and incubated at 37 ° C for 16 h (covering the upper mouth with a plastic film to prevent liquid evaporation). 3. Each tube was washed 3 times with water. 4. Move the beads into the corresponding measuring tube and measure the radioactivity count. Not suitable for the crowd Those without examination indications should not be examined. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.