sugar chain antigen 15-3

Cancer antigen 15-3, also known as carbohydrate antigen 15-3, is a carbohydrate antigen recognized by two monoclonal antibodies, which are derived from milk fatty acid and breast cancer cells, and are antigens related to malignant tumors such as breast cancer. It has a good diagnostic value for breast cancer and ovarian cancer, but it is not sensitive to early breast cancer. Cancer antigen 15-3 is also an indicator of postoperative follow-up and monitoring of tumor recurrence and metastasis. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Tips: Polyethylene tubes should be used when diluting samples. Normal value <28kU/L. Clinical significance The increase is mainly seen in breast cancer, the positive rate of metastatic breast cancer can reach 80%; liver cancer, colon cancer, pancreatic cancer, ovarian cancer, cervical cancer, and primary liver cancer also have different degrees of elevation. The positive rate of non-malignant tumors such as breast and ovary is generally less than 10%. High results may be diseases: breast cancer, ovarian cancer, liver cancer, colon cancer considerations 1, cancer antigen 15-3 is often used to evaluate the effectiveness of breast cancer treatment, predictive indicators of recurrence and metastasis. The increase of cancer antigen in patients with recurrence after surgery is earlier than the clinical symptoms, so the determination of cancer antigen 15-3 can predict the recurrence of breast cancer. 2. The sensitivity of the method is 0.2 u/ml, and the measurement range is 0-240 u/ml without cross-reaction with other tumor markers (CEA, CA-19-9 and CA-125). 3, the reagent must be stored in the refrigerator at 2 ~ 8 ° C, the storage period should not exceed 8 days (2 ~ 8 ° C) after removal. The ELSA tube must be sealed and stored at 2-8 °C. 4. Polyethylene tube should be used when the sample is diluted. 5. During the incubation process, the two washing processes require consistent operation, otherwise the experimental results will be affected. Inspection process 1. Immediately after blood collection, the test was performed using solid-phase two-site immunoradiometric analysis. 2. All reagents should be equilibrated at room temperature (18 ~ 25 ° C) for at least 30min, and mixed thoroughly before use. 3. Dilute the sample to be tested and the quality control serum: Take 2 μl of the sample or quality control serum into the corresponding test tube, then add 1000 μl of the diluted solution, and mix and test. Not suitable for the crowd Those without examination indications should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.