human acid ferritin

Human acid isoferritin (AIF) is an isomer of ferritin. It is rich in H subunits, and the isoelectric point is easy to form a polymer. Its properties are different from other ferritins and have different antigenic components. The general ferritin kit is aimed at the alkaline spleen ferritin, and AIP is mainly found in liver cancer tissues, cardiomyocytes and neutrophil cells, suggesting that AIF can be used as a tumor marker. Since the glycosylation modification of AIF protein in serum is more complete, and the myocardial infarction causes the cells to rupture and release into the blood, the AIF glycosylation is incomplete, and the blood circulation is very fast, and does not interfere with the determination of serum AIF. . Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Tips: Reagents and samples to be tested should be placed at room temperature to achieve equilibrium, and fully mixed before use. Normal value Serum male 154 μg / L; female 81.3 μg / L. Clinical significance The serological diagnosis of liver cancer mainly uses AFP. Because of the negative proportion of AFP negative and low level of liver cancer patients, the determination of AIF has certain auxiliary effects on the diagnosis of liver cancer. The positive rate of liver cancer was 75.9%, that of persistent hepatitis B was 17.2%, that of cirrhosis was 6.0%, and that of acute hepatitis was 2%. AIF had certain specificity for liver cancer. The diagnosis of liver cancer is a meaningful indicator. High results may be diseases: liver cancer, liver cirrhosis considerations 1. The method uses human myocardial AIF as antigen, AIF radioimmunoassay has no cross-reaction to AFP and CEA, and has a 21.9% cross-reaction to ferritin. 2. The kit should be stored at 2-8 °C, but should be prevented from freezing. 3. Reagents and samples to be tested should be placed at room temperature to achieve equilibrium, and mixed thoroughly before use. 4. Strictly control the reaction time. The standard curve, quality control serum and the sample to be tested should have the same reaction conditions. Inspection process The blood sample is taken immediately after the blood sample is collected. Check operation: Serum ferritin radioimmunoassay was measured using an unbalanced procedure. In the standard/test sample, ferritin and antibody are pre-reacted at appropriate temperature to form a complex, then the 125I-ferritin is added to continue the incubation, and finally the rapid double antibody (polyethylene glycol as a precipitant) is used to separate the sample. Binding state and free state of 125I-ferritin. Not suitable for the crowd Not suitable for people: those who do not have indications for examination should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.