p53 gene detection

The normal p53 gene encodes a 53 kD protein, which plays an important regulatory role in the cell cycle and has an inhibitory effect on cell carcinogenesis. When the gene undergoes point mutation, deletion and inactivation, it is transformed from wild type to mutant type, which promotes the occurrence and development of tumor. Basic Information Specialist Category: Oncology Inspection Category: Genetic Testing (DNA) Applicable gender: whether men and women apply fasting: not fasting Tips: Don't be nervous when checking, cooperate with the doctor's check. Normal value The gene has no mutation. Clinical significance The test is used for the study of various tumorigenesis mechanisms, tumor diagnosis and treatment, and prognosis evaluation. The loss of the p53 gene (tumor suppressor gene) causes the occurrence of various familial cancers. These different cancers include breast cancer, brain tumors, malignant sarcoma, bone cancer, etc. Many of them occur at a young age and are rare. Autosomal dominant genetic disease. The main features are early onset cancer (less than 45 years old), simultaneous multiple cancers or sarcomas. The determined diagnosis and treatment needs to be determined by genetic testing. The p53 gene mutation can be detected in tissue samples of 1.90% of SCLC patients, and about 60% of mutations in NSCLC patients. 2. Mutants have poor response to radiotherapy and chemotherapy, and are also prone to metastasis, which can be used as an indicator for therapeutic efficacy and prognosis. 3. If the p53 gene is frequently mutated in the early stage of tumorigenesis, it will contribute to the early diagnosis of tumors. 4. Wild-type p53 gene can inhibit the growth of cancer cells, and it is expected that the p53 gene mutant can achieve gene therapy by gene transfer technology. Precautions Specimen contamination is strictly avoided during the test. Inspection process Take a blood sample and then perform a genetic test. Not suitable for the crowd There are no absolute or relatively contraindicated people in this test. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.