K-ras gene detection (K-ras, p21)

The ras gene family has three genes associated with human tumors - H-ras, K-ras, and N-ras, which are located on chromosomes 11, 12, and 1, respectively. K-ras is also known as the p21 gene due to the 21kD ras protein. Among the ras genes, K-Ras has the greatest impact on human cancer. It is like a molecular switch. When it is normal, it can control the path of regulating cell growth. When an abnormality occurs, it leads to the continuous growth of cells and prevents the cells from self-destruction. Involved in intracellular signaling, when the K-ras gene is mutated, the gene is permanently activated, unable to produce normal ras protein, causing intracellular signal transduction disorder, cell proliferation uncontrolled and cancerous. Basic Information Specialist Category: Oncology Inspection Category: Genetic Testing (DNA) Applicable gender: whether men and women apply fasting: not fasting Tips: Keep a normal mindset. Normal value No mutations. Clinical significance Detection of K-ras gene mutation is an important indicator for understanding the status of oncogenes, understanding the developmental prognosis of various cancers, and the efficacy of radiotherapy and chemotherapy. 1. K-ras gene mutation occurs in the early stage of tumor malignant transformation, and the K-ras gene of primary and metastatic lesions is highly consistent. It is generally believed that the K-ras gene status does not change due to treatment. 2, K-ras gene mutations are found in 20% of non-small cell lung cancer (NSCLC), of which lung adenocarcinoma accounts for 30% to 50%. The K-ras mutation rate in patients with colorectal cancer is 30% to 35%. Precautions Specimen contamination is strictly avoided during the test. Inspection process Take small cells and then perform genetic testing. Not suitable for the crowd There are no absolute or relative contraindications to this test. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.