Apolipoprotein E Genotyping

Apolipoprotein E (ApoE) is an alkaline protein rich in arginine, and ApoE plays an important role in lipid metabolism. The common alleles of the apolipoprotein (Apo) E gene are divided into three types of ε2, ε3, and ε4. The detection of the ApoE genotype can be used as an indicator for clinical diagnosis and risk rate estimation. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Keep your mood steady and avoid irritability, excitement or sullenness. Normal value Apolipoprotein E is mainly present in CM, VLDL, IDL and part of HDL, and normal human plasma ApoE concentration is 0.03 to 0.05 g/L. The concentration of ApoE was positively correlated with plasma triglyceride content. Clinical significance Abnormal result Light microscopy and electron microscopy showed that the number of cells in the inner and outer nucleus of the ApoE-deficient mouse was significantly reduced, the nuclear chromatin in the outer nuclear layer was concentrated, the perinuclear vacuoles were formed, the glass membrane was thickened, the elastic layer was discontinuous or even the structure was unclear. AMD's fundus damage occurred. High results may be diseases: high triglycerideemia precautions Taboo before check-up: Keep your mood steady and avoid irritability, excitement or sullenness. Requirements for inspection: Please follow the doctor's advice and cooperate with the doctor. Inspection process The template DNA was extracted by sodium iodide method, and the gene sequence of 112 and 158 amino acids of ApoE gene was specifically amplified by polymerase chain reaction (PCR). The amplified product was digested with restriction endonuclease HhaI, polyacrylamide gel. After electrophoresis, a restriction fragment length polymorphism (RFIP) map of the cleavage site was observed. ApoE phenotypic detection can be performed by using VLDL as a sample or directly as a sample of serum (or plasma) by IEF electrophoresis analysis, two-way PAGE or immunoblotting. Not suitable for the crowd Inappropriate crowd: This test is not intended to screen the general population. It is used in very special circumstances and provides additional information to the doctor. No indications for examination, normal blood lipids should not be detected. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.