Apolipoprotein B (ApoB)

Apolipoprotein B100 is mainly synthesized in the liver and is the main structural protein of lipoproteins other than high-density lipoprotein, which transports lipids to extrahepatic tissues. Reduces the substantial lesions common in liver damage, hyperthyroidism, and low beta-lipoproteinemia. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduces the substantial lesions common in liver damage, hyperthyroidism, and low beta-lipoproteinemia. Normal value: Male: 0.43-1.28g/L Female: 0.42-1.12g/L Above normal: Increased in type II hyperlipidemia, cholestasis, coronary heart disease, kidney disease, cerebrovascular disease, hypothyroidism, psoriasis. negative: Positive: Tips: Actively cooperate with the doctor during the examination. Normal value Male 0.43 ~ 1.28g / L (43 ~ 128mg / dl); Female 0.42 ~ 1.12g / L (43 ~ 128mg / dl). Clinical significance Increased adults > l.0 g / liter is slightly higher; > 1.2g / L is significantly increased. Increased in type II hyperlipidemia, cholestasis, coronary heart disease, kidney disease, cerebrovascular disease, hypothyroidism, psoriasis. Reduces the substantial lesions common in liver damage, hyperthyroidism, and low beta-lipoproteinemia. Low results may be diseases: familial abnormal β-lipoproteinemia results may be high disease: coronary heart disease, primary hyperlipoproteinemia and xanthoma hyperplasia, hyperthyroidism, hyperlipidemia, thyroid dysfunction in the elderly, Cerebrovascular disease, psoriasis precautions In the last meal before blood draw, avoid high-fat food and drinking, and fasting for 12 hours, extract forearm venous blood. Inspection process (1) Pouring board: 10g/L agarose 10ml per plate, melt in boiling water bath, mix well, add 50μl apoAI antiserum and 8μl apoB antiserum when cold to 50~55°C (the amount depends on antiserum titer) ), quickly mix and pour on the glass plate preset on the water platform, cool and then placed at 4 ° C, after 20 min, punch holes, the hole is at the cathode end of the plate, the hole diameter is 3 mm, the hole spacing is at least 5 mm, and the hole capacity is 5 μl. Place the plate in the electrophoresis tank and bridge with filter paper. (2) Dilution of antigen: The calibration serum was diluted to 1:100, 1:150, 1:200, 1:300, and 1:400 (for calibration curve) with 0.15 mol/L NaCl solution, and the serum sample was 1: Diluted in 200, put the refrigerator at 4 °C for no more than 3 days. (3) Loading: 5 μl (accurate) of the diluted serum and specimens were taken in a low current state (10 mA/plate) and added to the agarose gel sample well. Each board has to do a series of standards. (4) Power-on: steady flow 24 mA / plate, terminal voltage 6 ~ 8V / cm, cooling with running water to maintain agarose 15 ° C, electrophoresis 3 ~ 4h. (5) Deproteinization and dry film: The agarose plate after electrophoresis was immersed in 0.15mol/L NaCl for 30min, and the film was placed on the polyester film, and dried by light pressure under a multi-layer filter paper. The moisture in the glue is then dried naturally with the filter paper, the film and the polyester film, or dried by a hot air blower. After drying, the film is naturally separated from the filter paper and the polyester film. (6) Dyeing: The agarose film was immersed in the staining solution for 20 to 30 minutes. (7) Decolorization: Soak the dyed film with a decolorizing solution until the rocket peak is clear, and the background is basically colorless. It can be clamped in two pieces of cellophane in water and can be stored for a long time after drying. It can also be soaked in running water to remove the background. Not suitable for the crowd People with normal blood lipids should not be tested. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.