Oxidase test

The oxidase test is a test in which the oxidase does not directly react with the oxidase reagent, but first oxidizes the cytochrome C, and then the oxidized cytochrome C oxidizes the p-phenylenediamine to produce a color reaction. The results of this test are related to the presence of cytochrome C. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: For the difference between Pseudomonas and Enterobacteriaceae bacteria, the latter is negative. Positive: It is used for the identification of the species of Neisseria, and the bacteria are positive. For the difference between Pseudomonas and Enterobacteriaceae, the former is positive. Moraxella, Alcaligenes and the like are all positive. Reminder: Avoid contact with iron-containing substances during the test to avoid false positives. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance 1. It is used for the identification of the species of Neisseria, and the bacteria are positive. In addition, it is also used for the difference between Pseudomonas and Enterobacteriaceae, the former is positive, while the latter is negative. Moraxella, Alcaligenes and the like are all positive. 2, abnormal results: Neisseria has nine pathogenic bacteria such as Neisseria meningitidis and Neisseria gonorrhoeae, causing epidemic cerebrospinal meningitis. 3, upper respiratory symptoms, nasopharyngitis and so on. Symptoms of systemic infection, high fever, bleeding, or bleeding. Meningitis symptoms, headache, vomiting, neck stiffness. Cause gonorrhea, causing neonatal gonorrhea conjunctivitis. Positive results may be diseases: colorectal cancer, large intestine malignant lymphoma, gonococcal peritonitis, gonococcal infection, gonorrhea precautions Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. 1 Avoid contact with iron-containing substances during the test to avoid false positives. 210g/L tetramethyl-p-phenylenediamine hydrochloride or 10g/L aqueous solution of tetramethyl-p-phenylenediamine hydrochloride is a colorless solution, which is easily oxidized and invalid in air. Therefore, new reagents should be replaced frequently and contained in brown bottles. If the reagent has turned dark blue, it should be discarded. Inspection process Reagents: 1 g of tetramethyl-p-phenylenediamine hydrochloride, 100 ml of water (stored in a brown bottle, kept in the refrigerator for 2 weeks). method: 1. Take a filter paper and take a little colony of the test bacteria. Then add a drop of tetramethyl-p-phenylenediamine reagent, only the filter paper is wet, not too wet, red is positive in 10s, delayed in 10-60s red, red in 60s, negative treatment, Iron can be used as a catalytic reagent, can not be used, can be taken from white gold wire or glass rod. 2. After dampening the filter paper with 1% tetramethyl-p-phenylenediamine solution, add an equal amount of 1% menaphthol solution (methyl naphthol 1 g, alcohol 100 ml), then apply the lawn moss, and the blue color appears. For a positive reaction, the time at which color appears is determined as in Method 1. 3. Soak the Xinhua No. 1 filter paper with 1% tetramethyl-p-phenylenediamine solution, air dry in the room, place it in a dark bottle with a rubber stopper, and store it in the refrigerator for several months. If it is stored for too long, the color is too Deep, the color is not obvious, it should not be used. Usage is the same as before. Not suitable for the crowd Inappropriate people: Generally there are no people who are not suitable. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.