blood pyruvate

Pyruvic acid is a product of the metabolic process of sugar metabolism and can be oxidized to carbon dioxide and water. Under normal circumstances, blood lactic acid and pyruvic acid maintain a certain ratio (about 9). When the body is in the state of hypoxia metabolism, pyruvic acid is reduced to lactic acid, so that the ratio increases, and the more severe the hypoxia, the higher the ratio. Serum pyruvate assays can be used to predict the severity of circulatory failure. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: The pyruvic acid in the blood is extremely unstable, and the blood is reduced 1 min after the blood is drawn out. The pyruvic acid in the supernatant of the precipitated protein of metaphosphate was stable at 4 ° C for 8 days. Normal value In the fasting state, the venous blood pyruvate concentration is 0.03 to 0.1 mmol/L (0.3 to 0.9 mg/dl). Clinical significance 1. Pyruvic acid is a product of the glycolytic pathway. Under normal conditions, it is oxidized to CO2 and water by the cycle of tricarboxylic acid, so that the ratio of lactic acid/pyruvate in blood is maintained at about 9. When the body is in anoxic state, pyruvic acid is reduced to lactic acid, the ratio increases, and the more severe the hypoxia, the higher the ratio. This ratio determination can be used to predict the severity of circulatory failure. Slight activity causes both lactic acid and pyruvic acid to rise at the same time, but the ratio does not change. 2, blood pyruvate determination is mainly used for the diagnosis of vitamin B1 deficiency. The pyrophosphate of vitamin B1 is a decarboxylated coenzyme when pyruvate is further oxidatively decomposed into acetyl-CoA in cells. When vitamin B1 is deficient, the oxidation of pyruvate in the body is impeded, and the content of pyruvic acid is increased. 3, increased blood pyruvate is also seen in diabetes, heart failure, diarrhea, severe liver damage, acute infections. High results may be diseases: Diabetes lactic acidosis, vitamin B1 deficiency, beriberi considerations 1. The pyruvic acid in the blood is extremely unstable, and the blood is reduced 1 min after the blood is drawn out. The pyruvic acid in the supernatant of the precipitated protein of metaphosphate was stable at 4 ° C for 8 days. 2. The pyruvic acid standard application solution must be freshly prepared daily, because pyruvic acid will polymerize, and the rate of enzymatic reaction of the polymer is different from that of the non-polymer. 3. When the ratio of lactic acid to pyruvic acid is determined, the ratio of the above spectrophotometric method to the method of pyruvic acid should be obtained. Inspection process The preparation of protein-free supernatant was the same as that of whole blood lactic acid, and it was carried out according to Table 1. Mix and incubate for 2 min at room temperature before reading the absorbance, then read the absorbance every 1 min until the reading is stable. Not suitable for the crowd Inappropriate people: Generally there are no people who are not suitable. Adverse reactions and risks 1. Infection: Pay attention to aseptic operation when collecting blood, avoid contamination of water and other parts at the blood collection site to avoid local infection. 2, bleeding: after the blood is given a full compression time, especially coagulopathy, bleeding tendency, to avoid local subcutaneous oozing, bruising and swelling.