serum lipoprotein-alpha

Lipoprotein alpha is synthesized in the liver and is a special lipoprotein composed of apolipoprotein A and apolipoprotein B through a disulfide bond. The composition is similar to low density lipoprotein. Its main function is to prevent blood clots from damaging and promote the formation of atherosclerosis. The continuous increase of lipoprotein alpha level is closely related to angina pectoris, myocardial infarction and cerebral hemorrhage. Because lipoprotein alpha is not significantly associated with hypertension, smoking, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, etc., it is considered an independent risk factor for coronary heart disease. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value Immunoturbidimetric turbidity method 0 ~ 1g / L. Clinical significance 1. Reduced in severe liver disease. 2, elevated in atherosclerosis, cerebral infarction, cerebral arteriosclerosis, acute myocardial infarction, acute rheumatoid arthritis, surgery and so on. High results may be diseases: atherosclerosis, cerebral infarction, acute myocardial infarction, acute abdominal-hyperlipidemia syndrome The level of lipoprotein α was related to human race and heredity. There was no significant difference between genders. The factors such as environment, diet and drugs had no significant effect on the level of lipoprotein α. Inspection process Immediately after the blood is taken from the vein, the test is performed. 1. Coated: polystyrene plate, 96 wells. The anti-apo(a)-IgG was diluted with a coating solution to a concentration of 10 μg/ml, 150 μg/ml per well, and overnight at 4 °C. 2. Washing and sealing: The small holes after coating are washed 3 times with washing liquid for 5 minutes each time. 0.15 ml of the blocking solution was added to each well, and the mixture was allowed to stand at 37 ° C for 30 minutes, and then washed 3 times with the washing solution. 3. Dilution: Dilute the reference serum 1:200, and dilute 8 tubes with dilution to make a standard curve. Serum samples can be diluted to 1:100 or 1:2000 for review. 4. Adding sample: Add 100 μl of diluted reference serum and specimen to each well, leave only blank solution for the blank well, and wash it with washing solution for 3 times at 37 °C for 1 hour. 5. Add the enzyme-labeled antibody (dilution depends on the antibody titer, for example 1:20000): 100 μl per well, 37 ° C for 1 h. 6. Color development: 100 μl of substrate solution was added to each well, and left at room temperature (25 ° C) for 15 min. 7. Stop the reaction: 50 μl of stop solution was added to each well. 8. Colorimetric: On the enzyme standard colorimeter, use the blank hole to calibrate the zero point color, the wavelength is 490 nm, and read the absorbance of each well (A). Not suitable for the crowd Inappropriate people: Generally there are no people who are not suitable. Adverse reactions and risks 1, local subcutaneous hemorrhage: after the blood collection should be pressed for a sufficient time, especially those with bleeding tendency, so as not to cause subcutaneous oozing and bruising due to no blood coagulation. 2, infection: attention to aseptic operation during venous blood collection, so as not to cause local infection.