Serum acid phosphatase (ACP)

Acid phosphatase is mainly distributed in the prostate, liver, spleen, milk, red blood cells, platelets and bones. The prostate is the most abundant, so the detection of serum acid phosphatase is mainly used for the diagnosis of prostate cancer. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: The serum should be separated in time, the specimen can not be hemolyzed, otherwise the measurement result is high. Normal value 1. Enzyme rate method (37 ° C) 0.11 ~ 6.0U / L. 2. Phosphate disodium sulphate method <5 gram units. 3. The thymol bismuth phosphate method is 0.02~0.49U/L. 4, α-naphthol phosphate method 0.2 ~ 3.8U / L. (Note the specific reference value depends on each laboratory.) Clinical significance 1. Raise 1 prostate disease. Prostate cancer, benign prostatic hyperplasia, malignant tumor prostate metastasis, prostate infarction, etc. 2 bone disease. Malignant bone metastasis (liver, stomach, breast, lung), osteosarcoma, Paget's disease (abnormal osteitis), bone marrow tumor, metabolic bone disease (hyperparathyroidism), multiple myeloma, Niemann -Pick disease (sphingomyelin reticuloendotheliosis syndrome) and the like. 3 hepatobiliary diseases. Liver cancer, cirrhosis, hepatitis, obstructive jaundice, etc. 4 blood diseases. Thrombocytopenia, chronic leukemia, Hodgkin's disease, polycythemia vera, venous thrombosis, etc. 5 kidney disease. Renal insufficiency (independent of the extent of azotemia), chronic glomerulonephritis. 2, lower No special cause of disease, after taking estrogen or adrenal cortex treatment, after testicular removal. High results may be diseases: liver cancer, chronic glomerulonephritis, benign prostatic hyperplasia, prostate cancer, secondary thrombocytosis 1. Serum and heparin anticoagulated plasma results were similar, and oxalate anticoagulated plasma results were low. 2, the serum should be separated in time, the specimen can not be hemolyzed, otherwise the measurement results are high. 3. The specimen is extremely unstable in the higher temperature and alkaline environment. If it cannot be measured in time, the serum should be frozen or acidified and stored in the refrigerator for one week. Serum acidification method: 1 ml serum plus 50 μl of 5 mol/L acetate buffer can reduce the pH to 5.4. Inspection process Operation method: operate according to Table 1. Mix, 37 ° C for 5 min, 405 nm with distilled water to zero, 1 cm optical path, read absorbance of A1, then set 37 ° C after 5 min, the colorimetric is A2. A2-A1 is the absorbance change value of the enzymatic reaction for 5 min. If measured by automatic analyzer, it can be programmed according to the main parameters: wavelength 405nm, 37 °C, sample to reagent volume ratio is 1:15, delay time 5min, linear time 10min, kinetic method. Not suitable for the crowd Inappropriate people: Generally there are no people who are not suitable. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.