Cerebrospinal fluid pathogen examination

It is of great significance to perform cerebrospinal fluid pathogen examination in patients with meningeal irritation and suspected nervous system infectious diseases. Direct microscopic examination of cerebrospinal fluid has clinical significance for cryptococcal meningitis and epidemic cerebrospinal meningitis (abbreviated as cerebral palsy); the smear of the ink has a new type of cryptococcal bacteria, which may be prompted by the new cryptococcal meningitis; Gram-negative, concave-faced dicocci can be initially reported as meningitis caused by Neisseria meningitidis; see acid-positive bacilli, suggesting tuberculous meningitis. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal, no pathogens. Positive: A positive indication is infected by a pathogen. Tips: Cerebrospinal fluid should be counted within 1 hour after collection. If it is left for too long, cell destruction, or precipitation and fibrin condensation, resulting in inaccurate counting. Specimens must be shaken before they can be dropped into the counting chamber, otherwise the test results will be affected. Normal value No pathogens. Clinical significance Common pathogenic microorganisms in cerebrospinal fluid include Staphylococcus aureus, hemolytic streptococcus, Streptococcus pneumoniae, Listeria monocytogenes, Neisseria meningitidis, Haemophilus influenzae, Cryptococcus neoformans, Candida albicans, and the like. Direct microscopic examination of cerebrospinal fluid has clinical significance for cryptococcal meningitis and epidemic cerebrospinal meningitis (abbreviated as cerebral palsy); the smear of the ink has a new type of cryptococcal bacteria, which may be prompted by the new cryptococcal meningitis; Gram-negative, concave-faced dicocci can be initially reported as meningitis caused by Neisseria meningitidis; see acid-positive bacilli, suggesting tuberculous meningitis. In addition, pathogens are positively isolated and cultured, and can be reported as meningitis or encephalitis caused by the pathogen or the like. Positive results may be diseases: Pseudomonas aeruginosa meningitis, pediatric pneumococcal meningitis, pediatric haemophilic influenza bacillus meningitis, suppurative meningitis, toxoplasmosis encephalopathy Cerebrospinal fluid should be counted within 1 hour after collection, such as long-term retention, cell destruction, or precipitation and fibrin condensation, resulting in inaccurate counting. Specimens must be shaken before they can be dropped into the counting chamber, otherwise the test results will be affected. Inspection process For fungal examination, take the cerebrospinal fluid specimen, centrifuge at 2000rpm for 10 minutes, smear the precipitate, add a drop of filtered fine ink, mix it and check it with a cover glass microscope. First observe with a low power microscope, if it is found that there is a circular light-transmitting small spot on a black background, and there is a round material with a cell size in the middle, that is, the structure is carefully observed by using a high power microscope, and the diameter of the new Cryptococcus is 5-20 μm, which is obvious. The thick sandwich is surrounded by a strong refraction and has sprouted spherical spores. Not suitable for the crowd Can not do cerebrospinal fluid puncture: 1, increased intracranial pressure has occurred in the cerebral palsy. 2, posterior fossa lesions associated with severe intracranial hypertension. 3, the puncture site has recently infected lesions. 4, sepsis or systemic infection. 5, shock, debilitation, and ill. Adverse reactions and risks Cerebrospinal fluid puncture can cause a decrease in intracranial pressure.